ISSN 10214437, Russian Journal of Plant Physiology, 2011, Vol. 58, No. 2, pp. 370–374. © Pleiades Publishing, Ltd., 2011.
Original Russian Text © O.V. Ozerinina, V.D. Tsydendambaev, 2011, published in Fiziologiya Rastenii, 2011, Vol. 58, No. 2, pp. 302–307.
Ionizing radiation is widely used in breeding pro
grams and genetic studies with plants, sea buckthorn
in particular, to produce promising cultivars with
desired properties [1–3].
irradiation of sea buckthorn seeds
was performed to improve descendant fruit chemical
composition, to increase the contents of oil, caro
tenoids, vitamin C, etc. and also to produce mutants
with other valuable traits: larger fruits, easy fruit har
vesting, low density of thorns, winter tolerance, resis
tance to diseases, and others [1, 2, 4–6]. Such treat
ment resulted in the changes in the fruit weight and
chemical composition in the first plant generation
), including seed oil content  and fruit hypantium
[8–10]. Earlier, for rapeseed , flax , sunflower
[13–15], and soysbean [16–18], it has been shown
that physical mutagens (Xrays and
affect the content of seed oil in
plants and its
FA composition in a dosedependent way.
The content of lipids and FAcomposition in sea
buckthorn seed oil as dependent on the dose of pre
irradiation and cultivar genotype has not
been analyzed previously. Such investigations would
be of a great interest in the connection with the fact
that, in particular parts of sea buckthorn fruit (seeds
and mesocarp), different systems of FA and triacylg
lycerol synthesis function .
The objective of this work was to study changes in
the content of lipids and the composition of their FAs
in the seeds of
sea buckthorn plants in response to
irradiation as dependent on the
irradiation dose and cultivar genotype.
MATERIALS AND METHODS
Experiments were performed with the two promis
ing sea buckthorn (
Vitaminnaya and Zyryanka belonging to Siberian cli
matype but differing in the genotype. Cv. Vitaminnaya
is of Altai breeding , whereas largefruit cv. Zyryanka
Effect of PreSowing
Irradiation of sea buckthorn Seeds
on the Content and Fatty Acid Composition of Total Lipids
in the Seeds of the First Plant Generation
O. V. Ozerinina and V. D. Tsydendambaev
Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, 127276 Moscow, Russia;
fax: 7 (499) 9778018; email: firstname.lastname@example.org
Received June 23, 2010
—Absolute content and FAcomposition of sea buckthorn (
L.) seed lipids
were studied. The seeds of cvs. Vitaminnaya and Zyryanka belonging to the Siberian climatype and also the
seeds of the first generation (M
) plants grown from the seeds subjected to presowing
the doses of 50 and 100 Gy (cv. Vitaminnaya) and 100, 250, and 500 Gy (cv. Zyryanka) were used in analyses.
In all treatments, irradiation resulted in the reduced seed weight in M
plants, which was sharper in cv. Vita
minnaya. In contrast, oil content declined strongly in cv. Zyryanka seeds, especially after irradiation with
500 Gy, whereas this index remained almost unchanged in cv. Vitaminnaya. Control and treated plants were
close by their FA qualitative composition and by the total content of unsaturated FAs (88–90%). Presowing
seed irradiation resulted in the rise of the unsaturation index of lipids and linolenic acid concentration; at
lower irradiation doses, this rise was more pronounced. With the increase of irradiation dose, the content of
linolenic acid reduced in both cultivars, whereas the content of linoleic acid increased. As distinct from cv.
Vitaminnaya, in cv. Zyryanka irradiation increased the content of linoleate and reduction in the level of oleate.
The conclusion is that presowing
irradiation of sea buckthorn seeds could affect substantially on the basic
quantitative indices characterizing seeds of M
plants. The range and direction of induced changes depend
on both the dose of irradiation and cultivar genotype.
Keywords: Hippophaë rhamnoides
irradiation, doses of irradiation, plants of the first generation, seeds, fatty
acids, linolenic acid.
: Fatty acids: 14:0—myristic; 15:0—pentade
canoic; 16:0—palmitic; 16:1—palmitoleic; 17:0—margarinic;
18:0—stearic; 18:1—oleic; 18:2—linoleic; 18:3—linolenic;
UI—unsaturation index; FAME—methyl ester of FA.