1021-4437/03/5005- $25.00 © 2003
Russian Journal of Plant Physiology, Vol. 50, No. 5, 2003, pp. 634–639. Translated from Fiziologiya Rastenii, Vol. 50, No. 5, 2003, pp. 710–715.
Original Russian Text Copyright © 2003 by Shein, Andreeva, Polyakova, Zrazhevskaya.
Various pathogens induce a rapid change in the con-
tent of phenolic compounds, which play a signiﬁcant
role in the pathological process in conifer cells . In
the infected conifer tissues, there is a tendency for an
increase in lignin content [2–4]. At the same time, data
on proanthocyanidin (PA) content are rather contradic-
tory [2, 5, 6]. Hydroxybenzoic acids play an important
role in conifer pathogenesis .
is a group of fungi wide-spread in nature.
species belong to saprophytic organ-
isms, which are clearly pathogenic toward plants. Many
species induce conifer seedling lodging .
The fact that
considerably changes the phe-
nolic compound metabolism was demonstrated for the
infection-resistant Scots pine seedlings .
The investigation of pathogenic properties of vari-
species showed that their virulence
depended on the developmental conditions of both the
plant and parasite . A genetic heterogeneity of the
seed material can complicate the interpretation of
results obtained with seedlings, but, in a callus culture,
this drawback is manifested to a lesser extent.
The objective of this work was an attempt to corre-
late the virulence of various
strains with the
content of phenolic compounds, such as lignin, PA, and
HBA, in the Scots pine callus cells treated with myce-
lium extracts from these strains.
species used were isolated either
from the seedling roots of Scots pine from various arbo-
reta of the logging-lumbering enterprises of Krasno-
yarsk krai or from the roots and trunks of pine forest
MATERIALS AND METHODS
The callus cultures of pine (
tiated from the hypocotyl segments were cultured in a
Murasighe and Skoog agar-solidiﬁed nutrient medium
supplemented with 0.1 mg/l 2,4-D, 0.5 mg/l BA, and
0.5 mg/l kinetin in the dark for 30 days at 26
ten days after transferring the calli to a fresh nutrient
medium in petri dishes (ﬁve calli per dish), each callus
was treated with 10
l of a solution containing 50
mycelium extract. After a 48-h-long dark
exposure, the callus was ﬁxed with boiling ethanol.
The fungi were isolated from pine roots and other
tissues, which were treated with a 0.5% KMnO
tion and then washed with sterile water. The plant mate-
rial was placed in a humid chamber. Mature fungal col-
onies were isolated. In order to obtain pure fungal cul-
tures, the colonies were repeatedly transferred to a
potato–agar nutrient medium containing streptomycin
species were identiﬁed using the
method of Bilai . Some
species, such as
Effect of Pine Callus Elicitation by the
of Various Pathogenicity on the Content of Phenolic Compounds
I. V. Shein*, O. N. Andreeva**, G. G. Polyakova*, and G. K. Zrazhevskaya*
*Sukachev Institute of Forestry, Siberian Division, Russian Academy of Sciences,
Akademgorodok, Krasnoyarsk, 660036 Russia;
fax: 7 (3912) 43-3686; e-mail: email@example.com
**Center for Forest Defense of the Krasnoyarsk krai, Krasnoyarsk
Received May 13, 2002
L.) callus culture was treated with the mycelium extracts from six
strains. Previously, pine seedlings were infected with a spore suspension in order to test the pathogenicity of
the used strains. Callus culture infection resulted in a decrease in the free proanthocyanidin (PA) and an increase
in bound PA content. After treating the calli with all strains except
, the lignin con-
tent became lower than the control one. The most considerable changes involved the
(HBA) content, and its greatest change was observed after treating the calli with
, when the HBA con-
g/g dry wt) exceeded fourfold the control one. There was a positive correlation (
between the HBA content in the callus culture cells treated with a fungal extract and the virulence of
strains. At the same time, there was an inverse correlation (
= –0.80) between the lignin content in a callus
culture and the fungal virulence; the latter did not affect the contents of both free and bound PA.
Key words: Pinus sylvestris - Fusarium - callus - seedlings - elicitor - virulence - phenolic compounds
: BA—benzyladenine; HBA—