Effect of Overexpression of Heterochromatin DNA-binding Protein Abp1p on Cell Growth and Minichromosome Loss Frequency in Cofactor D Mutants of Schizosaccharomyces pombe

Effect of Overexpression of Heterochromatin DNA-binding Protein Abp1p on Cell Growth and... Mitotic chromosome segregation is partly achieved by interaction between microtubules (MTs)and the kinetochores of sister chromatids. The precise mechanism of the interaction between kinetochores and MTs remains unclear. We studied this process in fission yeastSchizosaccharomyces pombe by analyzing interaction between genes encoding kinetochore components, such as DNA-binding protein Abp1, and genes whose protein products affect the dynamics of MTs, such as cofactor D of tubulin dimer assembly. Analysis of cell growth and minichromosome loss frequency has demonstrated that mutations in the gene of cofactor D, especially mutationtsm1-512, increase the rate of minichromosome loss and the sensitivity to changes in Abp1 concentration in cells compared to wild-type cells. This suggests that these mutants are defective in some specific, but still unknown aspect of kinetochore–MT interaction. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Effect of Overexpression of Heterochromatin DNA-binding Protein Abp1p on Cell Growth and Minichromosome Loss Frequency in Cofactor D Mutants of Schizosaccharomyces pombe

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Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2004 by MAIK “Nauka/Interperiodica”
Subject
Biomedicine; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1023/B:RUGE.0000013444.91644.f7
Publisher site
See Article on Publisher Site

Abstract

Mitotic chromosome segregation is partly achieved by interaction between microtubules (MTs)and the kinetochores of sister chromatids. The precise mechanism of the interaction between kinetochores and MTs remains unclear. We studied this process in fission yeastSchizosaccharomyces pombe by analyzing interaction between genes encoding kinetochore components, such as DNA-binding protein Abp1, and genes whose protein products affect the dynamics of MTs, such as cofactor D of tubulin dimer assembly. Analysis of cell growth and minichromosome loss frequency has demonstrated that mutations in the gene of cofactor D, especially mutationtsm1-512, increase the rate of minichromosome loss and the sensitivity to changes in Abp1 concentration in cells compared to wild-type cells. This suggests that these mutants are defective in some specific, but still unknown aspect of kinetochore–MT interaction.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Oct 18, 2004

References

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