Effect of Ibuprofen and Rofecoxib Transport Parameters in the Frog Corneal Epithelium

Effect of Ibuprofen and Rofecoxib Transport Parameters in the Frog Corneal Epithelium Effects of cyclooxygenase (COX) inhibitors on transport parameters of the frog corneal epithelium were studied. Epithelial cells of the intact cornea were impaled with microelectrodes. Under short-circuit current (Isc) conditions, 10?4 M ibuprofen (IBU) (non-specific COX inhibitor) or 5 × 10?5 M rofecoxib (COX-2 inhibitor) were added to the tear solution. With ibuprofen, Isc decreased by 1.0 from 3.1 mA/cm2; intracellular potential, Vo, depolarized by 14.2 from ?56.9 mV; IBU did not affect the transepithelial conductance, gt, or the apical membrane fractional resistance, fRo. With rofecoxib, Isc decreased by 0.9 from 4.3 mA/cm2; Vo depolarized by 18 from ?62.4 mV; gt significantly increased by 0.03 from 0.37 ms/cm2; and fRo decreased by 12 from 50. Basolateral membrane K+ and apical membrane Cl? partial conductances were studied by the ion substitution method. Depolarization of Vo by an increase in stromal K+ from 4 to 79 mM was smaller with IBU (17.5 mV) or rofecoxib (19.2 mV) than without the inhibitors (29.1 and 29.3 mV, respectively). Depolarization of Vo, by a decrease in tear Cl? from 81 to 8.1 mM, was abolished by the COX inhibitors. Decrease in Isc and Vo can be explained by a decrease in the K+ and Cl? conductances. Experiments with amphotericin B ruled out a major effect of the inhibitors on the Na+/K+ ATPase pump. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Effect of Ibuprofen and Rofecoxib Transport Parameters in the Frog Corneal Epithelium

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Publisher
Springer-Verlag
Copyright
Copyright © 2002 by Springer-Verlag New York Inc.
Subject
Life Sciences; Biochemistry, general; Human Physiology
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s00232-002-1032-6
Publisher site
See Article on Publisher Site

Abstract

Effects of cyclooxygenase (COX) inhibitors on transport parameters of the frog corneal epithelium were studied. Epithelial cells of the intact cornea were impaled with microelectrodes. Under short-circuit current (Isc) conditions, 10?4 M ibuprofen (IBU) (non-specific COX inhibitor) or 5 × 10?5 M rofecoxib (COX-2 inhibitor) were added to the tear solution. With ibuprofen, Isc decreased by 1.0 from 3.1 mA/cm2; intracellular potential, Vo, depolarized by 14.2 from ?56.9 mV; IBU did not affect the transepithelial conductance, gt, or the apical membrane fractional resistance, fRo. With rofecoxib, Isc decreased by 0.9 from 4.3 mA/cm2; Vo depolarized by 18 from ?62.4 mV; gt significantly increased by 0.03 from 0.37 ms/cm2; and fRo decreased by 12 from 50. Basolateral membrane K+ and apical membrane Cl? partial conductances were studied by the ion substitution method. Depolarization of Vo by an increase in stromal K+ from 4 to 79 mM was smaller with IBU (17.5 mV) or rofecoxib (19.2 mV) than without the inhibitors (29.1 and 29.3 mV, respectively). Depolarization of Vo, by a decrease in tear Cl? from 81 to 8.1 mM, was abolished by the COX inhibitors. Decrease in Isc and Vo can be explained by a decrease in the K+ and Cl? conductances. Experiments with amphotericin B ruled out a major effect of the inhibitors on the Na+/K+ ATPase pump.

Journal

The Journal of Membrane BiologySpringer Journals

Published: Dec 1, 2002

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