Effect of human papillomavirus type 16 E6 and E7 oncogenes on the activity of the transforming growth factor-β2 (TGF-β2) promoter

Effect of human papillomavirus type 16 E6 and E7 oncogenes on the activity of the transforming... The effect of the human papillomavirus type 16 (HPV 16) E6 and E7 proteins was studied on the transcriptional activity of the human transforming growth factor β2 (TGF-β) promoter in different cell lines. Luciferase tests were performed after co-transfection of cells with TGF-β2 reporter constructs and HPV 16 E6 or E7 expression vectors. HPV 16 E7, but not E6 significantly repressed TGF-β2 promoter activity in NIH/3T3 cells, which have wild-type p53 and pRb proteins. The repressive effect of HPV 16 E7 on the transcriptional activity of the TGF-β2 promoter could be localized to the promoter region −528 to −251 relative to the transcriptional start site. Ability of E7 to bind pRb was necessary to inhibit the TGF-β2 promoter. Over-expression of the transcription factor E2F-1 had an effect on the TGF-β2 promoter similar to that of E7, which may indicate that HPV 16 E7 represses the TGF-β2 promoter by releasing E2F from pRb. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Effect of human papillomavirus type 16 E6 and E7 oncogenes on the activity of the transforming growth factor-β2 (TGF-β2) promoter

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Publisher
Springer-Verlag
Copyright
Copyright © 2004 by Springer-Verlag/Wien
Subject
Biomedicine; Medical Microbiology; Virology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-004-0376-x
Publisher site
See Article on Publisher Site

Abstract

The effect of the human papillomavirus type 16 (HPV 16) E6 and E7 proteins was studied on the transcriptional activity of the human transforming growth factor β2 (TGF-β) promoter in different cell lines. Luciferase tests were performed after co-transfection of cells with TGF-β2 reporter constructs and HPV 16 E6 or E7 expression vectors. HPV 16 E7, but not E6 significantly repressed TGF-β2 promoter activity in NIH/3T3 cells, which have wild-type p53 and pRb proteins. The repressive effect of HPV 16 E7 on the transcriptional activity of the TGF-β2 promoter could be localized to the promoter region −528 to −251 relative to the transcriptional start site. Ability of E7 to bind pRb was necessary to inhibit the TGF-β2 promoter. Over-expression of the transcription factor E2F-1 had an effect on the TGF-β2 promoter similar to that of E7, which may indicate that HPV 16 E7 represses the TGF-β2 promoter by releasing E2F from pRb.

Journal

Archives of VirologySpringer Journals

Published: Dec 1, 2004

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