Effect of growth regulators on ginsenoside production in the cell culture of two ginseng species

Effect of growth regulators on ginsenoside production in the cell culture of two ginseng species Plants belonging to the genus Panax produce ginsenosides that possess pharmacological properties. The ability to synthesize these compounds is preserved in some cultured cells of ginseng. In this work, we used suspension cell cultures of two species of ginseng: Panax japonicus var. repens C. A. Mey and P. ginseng C. A. Mey. The first culture was grown on MS medium supplemented with α-NAA. After one subculturing, cell biomass increased 5–6 times with the level of ginsenosides being equal to 2.5–3.0% of dry weight. The second culture was grown on the same medium supplemented with 2,4-D. In this case, biomass increment was 3–5-fold, and ginsenosides were produced in trace amounts. Substitution of 2,4-D for NAA in suspension cell culture of P. japonicus brought about deterioration of growth characteristics, but the content and composition of ginsenosides was not changed. In the suspension cell culture of P. ginseng, substitution of NAA for 2,4-D decreased the rate of biomass accumulation and increased the extent of cell aggregation, with total content of ginsenosides increasing 25 times and their assortment being complete. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Effect of growth regulators on ginsenoside production in the cell culture of two ginseng species

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2010 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Sciences ; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443710030167
Publisher site
See Article on Publisher Site

Abstract

Plants belonging to the genus Panax produce ginsenosides that possess pharmacological properties. The ability to synthesize these compounds is preserved in some cultured cells of ginseng. In this work, we used suspension cell cultures of two species of ginseng: Panax japonicus var. repens C. A. Mey and P. ginseng C. A. Mey. The first culture was grown on MS medium supplemented with α-NAA. After one subculturing, cell biomass increased 5–6 times with the level of ginsenosides being equal to 2.5–3.0% of dry weight. The second culture was grown on the same medium supplemented with 2,4-D. In this case, biomass increment was 3–5-fold, and ginsenosides were produced in trace amounts. Substitution of 2,4-D for NAA in suspension cell culture of P. japonicus brought about deterioration of growth characteristics, but the content and composition of ginsenosides was not changed. In the suspension cell culture of P. ginseng, substitution of NAA for 2,4-D decreased the rate of biomass accumulation and increased the extent of cell aggregation, with total content of ginsenosides increasing 25 times and their assortment being complete.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: May 9, 2010

References

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