Effect of different serum concentrations on short term in vitro culture of goat testicular cells

Effect of different serum concentrations on short term in vitro culture of goat testicular cells ISSN 1062-3604, Russian Journal of Developmental Biology, 2016, Vol. 47, No. 5, pp. 269–277. © Pleiades Publishing, Inc., 2016. DEVELOPMENTAL BIOLOGY OF MAMMALS Effect of Different Serum Concentrations on Short Term in vitro Culture of Goat Testicular Cells a, b c Mehrnoosh Bahadorani *, Morteza S. Hosseini , Hassan Abbasi , b b Parvaneh Abedi , and Mohammad H. Nasr-Esfahani ** Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran Department of Biology, Faculty of Science, Nour Danesh Institute of Higher Education, Meymeh, Isfahan, Iran *e-mail: m.bahadorani87@gmail.com, bahadorani_m@iaufala.ac.ir **e-mail: mh.nasr-esfahani@royaninstitute.org Received March 21, 2016; in final form, April 25, 2016 Abstract—Proper in vitro culture and proliferation of spermatogonial stem cells (SSCs) are key tools to achieve functional potentials of SSCs. The fresh serum extracted has been successfully used to stimulate growth of spermatogonial stem cells in vitro. However, there is no report on the optimal concentration of serum for in vitro culture of goat SSCs. This study investigated this issue. Material and methods: Retrieved testicular cells were cultured in DMEM in presence of different 0 to 15% rd th serum for 7 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Developmental Biology Springer Journals

Effect of different serum concentrations on short term in vitro culture of goat testicular cells

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Publisher
Pleiades Publishing
Copyright
Copyright © 2016 by Pleiades Publishing, Inc.
Subject
Life Sciences; Developmental Biology; Animal Anatomy / Morphology / Histology
ISSN
1062-3604
eISSN
1608-3326
D.O.I.
10.1134/S1062360416050064
Publisher site
See Article on Publisher Site

Abstract

ISSN 1062-3604, Russian Journal of Developmental Biology, 2016, Vol. 47, No. 5, pp. 269–277. © Pleiades Publishing, Inc., 2016. DEVELOPMENTAL BIOLOGY OF MAMMALS Effect of Different Serum Concentrations on Short Term in vitro Culture of Goat Testicular Cells a, b c Mehrnoosh Bahadorani *, Morteza S. Hosseini , Hassan Abbasi , b b Parvaneh Abedi , and Mohammad H. Nasr-Esfahani ** Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran Department of Biology, Faculty of Science, Nour Danesh Institute of Higher Education, Meymeh, Isfahan, Iran *e-mail: m.bahadorani87@gmail.com, bahadorani_m@iaufala.ac.ir **e-mail: mh.nasr-esfahani@royaninstitute.org Received March 21, 2016; in final form, April 25, 2016 Abstract—Proper in vitro culture and proliferation of spermatogonial stem cells (SSCs) are key tools to achieve functional potentials of SSCs. The fresh serum extracted has been successfully used to stimulate growth of spermatogonial stem cells in vitro. However, there is no report on the optimal concentration of serum for in vitro culture of goat SSCs. This study investigated this issue. Material and methods: Retrieved testicular cells were cultured in DMEM in presence of different 0 to 15% rd th serum for 7

Journal

Russian Journal of Developmental BiologySpringer Journals

Published: Sep 24, 2016

References

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