ISSN 1062-3604, Russian Journal of Developmental Biology, 2016, Vol. 47, No. 5, pp. 269–277. © Pleiades Publishing, Inc., 2016. DEVELOPMENTAL BIOLOGY OF MAMMALS Effect of Different Serum Concentrations on Short Term in vitro Culture of Goat Testicular Cells a, b c Mehrnoosh Bahadorani *, Morteza S. Hosseini , Hassan Abbasi , b b Parvaneh Abedi , and Mohammad H. Nasr-Esfahani ** Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran Department of Biology, Faculty of Science, Nour Danesh Institute of Higher Education, Meymeh, Isfahan, Iran *e-mail: email@example.com, firstname.lastname@example.org **e-mail: email@example.com Received March 21, 2016; in final form, April 25, 2016 Abstract—Proper in vitro culture and proliferation of spermatogonial stem cells (SSCs) are key tools to achieve functional potentials of SSCs. The fresh serum extracted has been successfully used to stimulate growth of spermatogonial stem cells in vitro. However, there is no report on the optimal concentration of serum for in vitro culture of goat SSCs. This study investigated this issue. Material and methods: Retrieved testicular cells were cultured in DMEM in presence of different 0 to 15% rd th serum for 7
Russian Journal of Developmental Biology – Springer Journals
Published: Sep 24, 2016
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