Effect of CTXφ prophage deletion in cholera agent on expression of regulatory genes controlling virulence and biofilm formation

Effect of CTXφ prophage deletion in cholera agent on expression of regulatory genes controlling... This work represents the results of comparative analysis of the nucleotide sequences in the chromosomal region containing CTXφ prophage in isogenic toxigenic (Tox+) and nontoxigenic (Tox–) strains of Vibrio cholerae biovar El Tor. It is shown that spontaneous mutants which have identical Tox– phenotype are formed either owing to the precise elimination of CTXφ prophage from the chromosome (deletion size is 6.9 kb) or owing to imprecise elimination with the additional loss of two other prophages (RS1φ and TLCφ), which are adjacent to CTXφ prophage (deletion size is 17.4 kb). It is revealed that, in the nontoxigenic mutants, CTXφ deletion initiates simultaneous change in several phenotypic properties, which are associated with virulence or biofilm formation, such as colonizing ability, HA/P production, VPS, and motility. For the first time, it is stated that the cause of the pleiotropic effect of the CTXφ deletion is a cascade decrease in the transcription levels of seven regulatory genes (toxR, aphA, tcpP, tcpH, toxT, vpsT, vpsR) which control virulence and biofilm formation processes in the cholera agent. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Effect of CTXφ prophage deletion in cholera agent on expression of regulatory genes controlling virulence and biofilm formation

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Publisher
Pleiades Publishing
Copyright
Copyright © 2017 by Pleiades Publishing, Inc.
Subject
Biomedicine; Human Genetics; Animal Genetics and Genomics; Microbial Genetics and Genomics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795417020119
Publisher site
See Article on Publisher Site

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