This work represents the results of comparative analysis of the nucleotide sequences in the chromosomal region containing CTXφ prophage in isogenic toxigenic (Tox+) and nontoxigenic (Tox–) strains of Vibrio cholerae biovar El Tor. It is shown that spontaneous mutants which have identical Tox– phenotype are formed either owing to the precise elimination of CTXφ prophage from the chromosome (deletion size is 6.9 kb) or owing to imprecise elimination with the additional loss of two other prophages (RS1φ and TLCφ), which are adjacent to CTXφ prophage (deletion size is 17.4 kb). It is revealed that, in the nontoxigenic mutants, CTXφ deletion initiates simultaneous change in several phenotypic properties, which are associated with virulence or biofilm formation, such as colonizing ability, HA/P production, VPS, and motility. For the first time, it is stated that the cause of the pleiotropic effect of the CTXφ deletion is a cascade decrease in the transcription levels of seven regulatory genes (toxR, aphA, tcpP, tcpH, toxT, vpsT, vpsR) which control virulence and biofilm formation processes in the cholera agent.
Russian Journal of Genetics – Springer Journals
Published: Apr 9, 2017
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
All the latest content is available, no embargo periods.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud