Effect of Cryoconservation on Seed Germination of Rare Tropical Orchids

Effect of Cryoconservation on Seed Germination of Rare Tropical Orchids In search of a procedure for cryoconservation, seeds of five species of tropical orchids and of one intergeneric hybrid were germinatedin vitroas depended on illumination. The other portion of seeds was frozen in liquid nitrogen (–196°C) and germinated after thawing. The viability of seeds, the percentage and rate of germination, as well as the growth rate of the developed protocorms were evaluated. At early stages of development, light inhibited growth in most species examined. The storage of seeds in liquid nitrogen had no negative effect on growth and development of protocorms and juvenile plants. The feasibility of the conservation of mature seeds of some tropical orchids by freezing in liquid nitrogen was demonstrated, which offers opportunities for creating a cryobank of orchids. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Effect of Cryoconservation on Seed Germination of Rare Tropical Orchids

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Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2001 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1023/A:1012520927743
Publisher site
See Article on Publisher Site

Abstract

In search of a procedure for cryoconservation, seeds of five species of tropical orchids and of one intergeneric hybrid were germinatedin vitroas depended on illumination. The other portion of seeds was frozen in liquid nitrogen (–196°C) and germinated after thawing. The viability of seeds, the percentage and rate of germination, as well as the growth rate of the developed protocorms were evaluated. At early stages of development, light inhibited growth in most species examined. The storage of seeds in liquid nitrogen had no negative effect on growth and development of protocorms and juvenile plants. The feasibility of the conservation of mature seeds of some tropical orchids by freezing in liquid nitrogen was demonstrated, which offers opportunities for creating a cryobank of orchids.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 10, 2004

References

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