Effect of copper ions on genetic variability in two maize callus lines of different ages

Effect of copper ions on genetic variability in two maize callus lines of different ages Variability of RAPD and ISSR markers was examined in two callus lines of maize (Zea mays L., lines ARm15 and ARm22), each obtained from one embryo and grown under standard conditions and on the medium supplemented with copper ions. The callus of ARm15 line was cultured in vitro for 8 months; the callus of ARm22 line, for 2 years. After these periods, the callus lines became genetically heterogeneous: the variability of DNA fragments was 13.3% in ARm15 and 15.0% in ARm22. Incubation in stress conditions on the medium supplemented with copper ions was accompanied by the decrease in the percentage of polymorphic DNA fragments. When the primer QR2 was used with DNA of ARm15 line grown on a copper-containing medium, a new amplicon was detected that was not observed in the original culture. The pattern of genomic changes was independent of the copper concentration in the medium and of the age of cultured cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Effect of copper ions on genetic variability in two maize callus lines of different ages

Loading next page...
Pleiades Publishing
Copyright © 2015 by Pleiades Publishing, Ltd.
Life Sciences; Plant Physiology; Plant Sciences
Publisher site
See Article on Publisher Site


You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial