Variability of RAPD and ISSR markers was examined in two callus lines of maize (Zea mays L., lines ARm15 and ARm22), each obtained from one embryo and grown under standard conditions and on the medium supplemented with copper ions. The callus of ARm15 line was cultured in vitro for 8 months; the callus of ARm22 line, for 2 years. After these periods, the callus lines became genetically heterogeneous: the variability of DNA fragments was 13.3% in ARm15 and 15.0% in ARm22. Incubation in stress conditions on the medium supplemented with copper ions was accompanied by the decrease in the percentage of polymorphic DNA fragments. When the primer QR2 was used with DNA of ARm15 line grown on a copper-containing medium, a new amplicon was detected that was not observed in the original culture. The pattern of genomic changes was independent of the copper concentration in the medium and of the age of cultured cells.
Russian Journal of Plant Physiology – Springer Journals
Published: Jan 4, 2015
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