Effect of BCL-2 on Harringtonine-induced apoptosis and intracellular Ca2+ mobilization in human leukemia HL-60 cells

Effect of BCL-2 on Harringtonine-induced apoptosis and intracellular Ca2+ mobilization in human... Harringtonine (HT), a kind of anticancer drug isolated from Chinese herb Cephalotaxus hainanensis Li, has been used in the clinical treatment of human glanulocytic leukemia and chromic myelocytic leukemia. In this study, we investigated the effect of Bcl-2 on HT-induced apoptosis and Ca2+ mobilization in human leukemia HL-60 cells. 1 μg/ml HT induced the apoptosis of HL-60/Neo cells in a time-dependent manner; while 1 μg/ml HT failed to induce the apoptosis of HL-60/ Bcl-2 cells. HT-, A23187- (a Ca2+ ionophore), Carbonyl cyanide m-chlorophenylhydrazone (CCCP,a specific releaser of Ca2+ from mitochondria) and thapsigargin- (an inhibitor of endoplasmic reticulum Ca2+> -ATPase) induced changes in [Ca2+]i were monitored by using Fluo 3-AM with confocal laser scanning microscopy. The results demonstrated that HL-60 cells with enforced expression of Bcl-2 (HL-60/Bcl-2 cells) had increased Ca2+ permeability and increased intracellular Ca2+ store in comparison with HL-60 cells with negative control vectors (HL-60/Neo cells), suggesting that Bcl-2 might prevent HT-induced apoptosis by increased Ca2+ permeability and increased intracellular Ca2+ buffering capacity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Research on Chemical Intermediates Springer Journals

Effect of BCL-2 on Harringtonine-induced apoptosis and intracellular Ca2+ mobilization in human leukemia HL-60 cells

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Publisher
Brill Academic Publishers
Copyright
Copyright © 2001 by VSP 2001
Subject
Chemistry; Inorganic Chemistry; Physical Chemistry
ISSN
0922-6168
eISSN
1568-5675
D.O.I.
10.1163/156856701753536714
Publisher site
See Article on Publisher Site

Abstract

Harringtonine (HT), a kind of anticancer drug isolated from Chinese herb Cephalotaxus hainanensis Li, has been used in the clinical treatment of human glanulocytic leukemia and chromic myelocytic leukemia. In this study, we investigated the effect of Bcl-2 on HT-induced apoptosis and Ca2+ mobilization in human leukemia HL-60 cells. 1 μg/ml HT induced the apoptosis of HL-60/Neo cells in a time-dependent manner; while 1 μg/ml HT failed to induce the apoptosis of HL-60/ Bcl-2 cells. HT-, A23187- (a Ca2+ ionophore), Carbonyl cyanide m-chlorophenylhydrazone (CCCP,a specific releaser of Ca2+ from mitochondria) and thapsigargin- (an inhibitor of endoplasmic reticulum Ca2+> -ATPase) induced changes in [Ca2+]i were monitored by using Fluo 3-AM with confocal laser scanning microscopy. The results demonstrated that HL-60 cells with enforced expression of Bcl-2 (HL-60/Bcl-2 cells) had increased Ca2+ permeability and increased intracellular Ca2+ store in comparison with HL-60 cells with negative control vectors (HL-60/Neo cells), suggesting that Bcl-2 might prevent HT-induced apoptosis by increased Ca2+ permeability and increased intracellular Ca2+ buffering capacity.

Journal

Research on Chemical IntermediatesSpringer Journals

Published: Oct 7, 2004

References

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