ISSN 10214437, Russian Journal of Plant Physiology, 2012, Vol. 59, No. 2, pp. 212–216. © Pleiades Publishing, Ltd., 2012.
Original Russian Text © I.A. Prokopiev, F.V. Filippova, A.A. Shein, 2012, published in Fiziologiya Rastenii, 2012, Vol. 59, No. 2, pp. 238–243.
Due to increased anthropogenic pollution of the
environment, at present the significance of the
assessement of biological system tolerance to stressors
of different nature and intensity is increased .
Dust pollution exerts double action on living
organisms: mechanical action and that of chemical
compounds present in dust. Chronic anthropogenic
pollution leads to accumulation of great amounts of
heavy metal ions (HMI) in the system soil–plant [2,
3]. An elevated HMI concentration in the cells
enhances ROS generation, which, in its turn, can lead
to oxidative stress development and subsequent death
of plant cells . Longterm action of anthropogenic
pollutants can be not only toxic to plants but also
induce adaptive physiological,cytological, and bio
chemical responses. It was shown that accumulation
of phenolic compounds and tocopherols and also acti
vation of antioxidant enzymes could substantially
improve plant tolerance to HMI toxicity [4–6].
One of the most important directions of investiga
tions of ecosystems in Central Yakutia is the study of
the influence of anthropogenic pollutuion on plant
organisms that grow under conditions of arid
extremely continental climate and permafrost. At the
same time, in plants grown under these conditions,
specific adaptive physiological, cytological, and bio
chemical responses can form [2, 7].
The objection of this work was to study the effect of
anthropogenic pollution on physiological, cytologi
cal, and biochemical characteristics of spear saltbush
seed progeny inhabiting Central Yakutia.
MATERIALS AND METHODS
Spear saltbrush (
L.) is an annual
herbaceous plant belonging to the family Chenopodi
aceae. It grows along roads, on ruderal sites, and saline
meadows . The choice of
by the fact that this plant belongs to polyzonal, meso
xerophyte, facultatively synanthropic species in the great
number of individuals in the sites of compact growth, and
it manifests a rather high seed productivity .
seeds were sampled in 2009 on the terri
tory of Yakutsk from wild plants impacted by anthro
pogenic pollution, e.g., growing at a distance of 5 m
from the roadway with a heavy load (
= 9), where the
level of dust pollution was
Control seeds were collected from plamts growing away
from polluted areas, at a distance of 1 km away from the
= 10) and at the dust level of
day). Seeds were sampled individually
from each plant.
Effect of Anthropogenic
Pollution with Dust Containing Heavy
Metals on Seed Progeny of Spear Saltbush
I. A. Prokopiev, F. V. Filippova, and A. A. Shein
Institute of Biological Problems of Cryolitozone, Siberian Branch, Russian Academy of Sciences, pr. Lenina 41, Yakutsk,
Received July 7, 2011
—The influence of anthropogenic pollution with dust containing heavy metals on changes in phys
iological, cytological, and biochemical characteristics of spear saltbrush (
L.) seed progeny
grown under conditions of extreme continental climate and permafrost in Central Yakutia was studied.
pollution with such dust of the parent habitat led to the decrease in physiological character
istics of seed progeny: germinability, root length. In this case, there was an increase in the lipid peroxidation
intensity, the contents of lowmolecular antioxidants and activity of antioxidant enzymes, and also the rate
of moleculargenetic processes (replication and reparation of DNA, protein translation) against the back
grounds of a relatively small increase in mitotic activity in seedlings grown from produced seeds as compared
with control. This can be considered as adaptive responses of
to the action of chronic anthropogenic
environmental stressor in a certain range of its intensity.
Keywords: Atriplex patula
, anthropogenic pollution, heavy metals, stress, antioxidant systems, adaptation.
: Car—carotenoids; CAT—catalase; Chl—chloro
phyll; HMI—heavy metal ions; ICPMS—inductively coupled
plasmamass spectrometry; LHC—lightharvesting complex;
LMAO—lowmolecular antioxidants; MI—mitotic index;
POD—peroxidase; SOD—superoxide dismutase.