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Ectopic expression of GhCOBL9A, a cotton glycosyl-phosphatidyl inositol-anchored protein encoding gene, promotes cell elongation, thickening and increased plant biomass in transgenic Arabidopsis

Ectopic expression of GhCOBL9A, a cotton glycosyl-phosphatidyl inositol-anchored protein encoding... Cellulose is a major component of plant cell walls and is necessary for plant morphogenesis and biomass. COBL (COBRA-Like) proteins have been shown to be key regulators in the orientation of cell expansion and cellulose crystallinity status. To clarify the role of a cotton COBL gene, GhCOBL9A, we conducted the ectopic expression and functional analysis in Arabidopsis. Previous study showed that GhCOBL9A was preferentially expressed during secondary cell wall biosynthesis in cotton fibers, and showed a significant co-expression pattern with cellulose synthase genes. Here, we detected that overexpression of GhCOBL9A induced the up-regulation of genes related to cellulose synthesis and enhanced the cellulose deposition. As a result, GhCOBL9A transgenic plants displayed increased hypocotyl and root lengths in early development, and cell wall thickening at the SCW stage. Notably, overexpression of GhCOBL9A led to an erect, robust-stature phenotype and brought higher biomass in mature plants. In addition, overexpression of GhCOBL9A in Arabidopsis AtCOBL4 mutants, a paralogous gene of GhCOBL9A, also led to a stronger growth potential, but the Atcobl4 mutant phenotype could not be rescued, implying the functional divergence of GhCOBL9A and AtCOBL4 paralogs. Taken together, these results suggest that overexpression of GhCOBL9A contributes to plant cell elongation and thickening, and increased biomass, which provides references for further utilizing GhCOBL9A to improve yield and quality traits in cotton and other species. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Genetics and Genomics Springer Journals

Ectopic expression of GhCOBL9A, a cotton glycosyl-phosphatidyl inositol-anchored protein encoding gene, promotes cell elongation, thickening and increased plant biomass in transgenic Arabidopsis

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Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer-Verlag GmbH Germany, part of Springer Nature
Subject
Life Sciences; Plant Genetics and Genomics; Human Genetics; Microbial Genetics and Genomics; Animal Genetics and Genomics; Biochemistry, general
ISSN
1617-4615
eISSN
1617-4623
DOI
10.1007/s00438-018-1452-3
pmid
29869696
Publisher site
See Article on Publisher Site

Abstract

Cellulose is a major component of plant cell walls and is necessary for plant morphogenesis and biomass. COBL (COBRA-Like) proteins have been shown to be key regulators in the orientation of cell expansion and cellulose crystallinity status. To clarify the role of a cotton COBL gene, GhCOBL9A, we conducted the ectopic expression and functional analysis in Arabidopsis. Previous study showed that GhCOBL9A was preferentially expressed during secondary cell wall biosynthesis in cotton fibers, and showed a significant co-expression pattern with cellulose synthase genes. Here, we detected that overexpression of GhCOBL9A induced the up-regulation of genes related to cellulose synthesis and enhanced the cellulose deposition. As a result, GhCOBL9A transgenic plants displayed increased hypocotyl and root lengths in early development, and cell wall thickening at the SCW stage. Notably, overexpression of GhCOBL9A led to an erect, robust-stature phenotype and brought higher biomass in mature plants. In addition, overexpression of GhCOBL9A in Arabidopsis AtCOBL4 mutants, a paralogous gene of GhCOBL9A, also led to a stronger growth potential, but the Atcobl4 mutant phenotype could not be rescued, implying the functional divergence of GhCOBL9A and AtCOBL4 paralogs. Taken together, these results suggest that overexpression of GhCOBL9A contributes to plant cell elongation and thickening, and increased biomass, which provides references for further utilizing GhCOBL9A to improve yield and quality traits in cotton and other species.

Journal

Molecular Genetics and Genomics – Springer Journals

Published: Jun 5, 2018

References

  • Polyploidy and genome evolution in plants
    Adams, KL; Wendel, JF
  • A membrane-associated form of sucrose synthase and its potential role in synthesis of cellulose and callose in plants
    Amor, Y; Haigler, CH; Johnson, S; Wainscott, M; Delmer, DP
  • Root development in Arabidopsis: four mutants with dramatically altered root morphogenesis
    Benfey, PN; Linstead, PJ; Roberts, K; Schiefelbein, JW; Hauser, MT; Aeschabacher, RA
  • COBRA-LIKE2, a member of the glycosylphosphatidylinositol-anchored COBRA-LIKE family, plays a role in cellulose deposition in Arabidopsis seed coat mucilage secretory cells
    Ben-Tov, D; Abraham, Y; Stav, S; Thompson, K; Loraine, A; Elbaum, R; Souza, A; Pauly, M; Kieber, JJ; Harpaz-Saad, S
  • Combining expression and comparative evolutionary analysis. The COBRA gene family
    Brady, SM; Song, S; Dhugga, KS; Rafalski, JA; Benfey, PN
  • Identification of novel genes in Arabidopsis involved in secondary cell wall formation using expression profiling and reverse genetics
    Brown, DM; Zeef, LAH; Ellis, J; Goodacre, R; Turner, SR
  • Brittle stalk 2 encodes a putative glycosylphosphatidylinositol-anchored protein that affects mechanical strength of maize tissues by altering the composition and structure of secondary cell walls
    Ching, A; Dhugga, KS; Appenzeller, L; Meeley, R; Bourret, TM; Howard, RJ; Rafalski, A
  • Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
    Clough, SJ; Bent, AF
  • Molecular characterization, expression pattern, and function analysis of the OsBC1L family in rice
    Dai, XX; You, CJ; Wang, L; Chen, GX; Zhang, QF; Wu, CY
  • OsBC1L4 encodes a COBRA-like protein that affects cellulose synthesis in rice
    Dai, XX; You, CJ; Chen, GX; Li, XH; Zhang, QF; Wu, CY
  • What do we really know about cellulose biosynthesis in higher plants?
    Guerriero, G; Fugelstad, J; Bulone, V
  • The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants
    Gutierrez, L; Mauriat, M; Guénin, S; Pelloux, J; Lefebvre, JF; Louvet, R; Rusterucci, C; Moritz, T; Guerineau, F; Bellini, C; Wuytswinkel, O
  • Cotton fiber: a powerful single-cell model for cell wall and cellulose research
    Haigler, CH; Betancur, L; Stiff, MR; Tuttle, JR
  • The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation
    Hajdukiewicz, P; Svab, Z; Maliga, P
  • Conditional root expansion mutants of Arabidopsis
    Hauser, MT; Morikami, A; Benfey
  • Using CLUSTAL for multiple sequence alignments
    Higgins, DG; Thompson, JD; Gibson, TJ
  • The maize (Zea mays L.) roothairless 3 gene encodes a putative GPI-anchored, monocot-specific, COBRA-like protein that significantly affects grain yield
    Hochholdinger, F; Wen, TJ; Zimmermann, R; Chimot-Marolle, P; Costa e Silva, O; Bruce, W; Lamkey, KR; Wienand, U; Schnable, PS
  • The plant cell wall: a complex and dynamic structure as revealed by the responses of genes under stress conditions
    Houston, K; Tucker, MR; Chowdhury, J; Shirley, N; Little, A
  • The use of an optical brightener in the study of plant structure
    Hughes, J; McCully, ME
  • Navigating the transcriptional roadmap regulating plant secondary cell wall deposition
    Hussey, SG; Mizrachi, E; Creux, NM; Myburg, AA
  • Extraction of total RNA in cotton tissues with CTAB-acidic phenolic method
    Jiang, JX; Zhang, TZ
  • Cotton fiber growth in planta and in vitro: models for plant cell elongation and cell wall biogenesis
    Kim, HJ; Triplett, BA
  • Plant cellulose synthesis: CESA proteins crossing kingdoms
    Kumar, M; Turner, S
  • Secondary cell walls: biosynthesis and manipulation
    Kumar, M; Campbell, L; Turner, S
  • Functional analysis of cellulose synthase (CESA) protein class specificity
    Kumar, M; Atanassov, I; Turner, S
  • BRITTLE CULM1, which encodes a COBRA-like protein, affects the mechanical properties of rice plants
    Li, YH; Qian, Q; Zhou, YH; Yan, MX; Sun, L; Zhang, M; Fu, ZM; Wang, YH; Han, B; Pang, XM; Chen, MS; Li, JY
  • Cellulose synthesis and its regulation
    Li, SD; Bashline, L; Lei, L; Gu, Y
  • Brittle Culm1, a COBRA-like protein, functions in cellulose assembly through binding cellulose microfibrils
    Liu, L; Shang-Guan, K; Zhang, B; Liu, X; Yan, M; Zhang, L; Shi, Y; Zhang, M; Qian, Q; Li, J; Zhou, Y
  • Analysis of relative gene expression data using real-time quantitative PCR and the 2–△△CT method
    Livak, KJ; Schmittgen, TD
  • CDD/SPARCLE: functional classification of proteins via subfamily domain architectures
    Marchler-Bauer, A; Bo, Y; Han, L; He, J; Lanczycki, CJ; Lu, S; Chitsaz, F; Derbyshire, MK; Geer, RC; Gonzales, NR; Gwadz, M; Hurwitz, DI; Lu, F; Marchler, GH; Song, JS; Thanki, N; Wang, Z; Yamashita, RA; Zhang, D; Zheng, C; Geer, LY; Bryant, SH
  • The NAC transcription factors NST1 and NST2 of Arabidopsis regulate secondary wall thickenings and are required for anther dehiscence
    Mitsuda, N; Seki, M; Shinozaki, K; Ohme-Takagi, M
  • A plasma membrane-bound putative endo-1,4-beta-D-glucanase is required for normal wall assembly and cell elongation in Arabidopsis
    Nicol, F; His, I; Jauneau, A; Vernhettes, S; Canut, H; Hofte, H
  • Comprehensive analysis of the COBRA-Like (COBL) gene family in Gossypium identifies two COBLs potentially associated with fiber quality
    Niu, E; Shang, X; Cheng, C; Bao, J; Zeng, Y; Cai, C; Du, X; Guo, W
  • Polychromatic staining of plant cell walls by toluidine blue O
    O’Brien, TP; Feder, N; McCully, ME
  • A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis
    Paterson, AH; Brubaker, CL; Wendel, JF
  • Complementary techniques: validation of gene expression data by quantitative real time PCR
    Provenzano, M; Mocellin, S
  • The cellulose synthase superfamily
    Richmond, TA; Somerville, CR
  • The COBRA family of putative GPI-anchored proteins in Arabidopsis. a new fellowship in expansion
    Roudier, F; Schindelman, G; DeSalle, R; Benfey, PN
  • COBRA, an Arabidopsis extracellular glycosyl-phosphatidyl inositol-anchored protein, specifically controls highly anisotropic expansion through its involvement in cellulose microfibrils orientation
    Roudier, F; Fernandez, AG; Fujita, M; Himmelspach, R; Borner, GH; Schindelman, G; Song, S; Baskin, TI; Dupree, P; Wasteneys, GO; Benfey, PN
  • Chitinase-like1/POM-POM1 and its homolog CTL2 are glucan-interacting proteins important for cellulose biosynthesis in Arabidopsis
    Sánchez-Rodríguez, C; Bauer, S; Hématy, K; Saxe, F; Ibáñez, AB; Vodermaier, V; Konlechner, C; Sampathkumar, A; Rüggeberg, M; Aichinger, E; Neumetzler, L; Burgert, I; Somerville, C; Hauser, MT; Persson, S
  • Glycosyltransferases and cell wall biosynthesis: novel players and insights
    Scheible, WR; Pauly, M
  • COBRA encodes a putative GPI-anchored protein, which is polarly localized and necessary for oriented cell expansion in Arabidopsis
    Schindelman, G; Morikami, A; Jung, J; Baskin, TI; Carpita, NC; Derbyshire, P; McCann, MC; Benfey, PN
  • Down-regulation of the cotton endo-1,4-β-glucanase gene KOR1 disrupts endosperm cellularization, delays embryo development, and reduces early seedling vigour
    Shang, X; Chai, Q; Zhang, Q; Jiang, J; Zhang, T; Guo, W; Ruan, Y
  • Cellulose synthesis in higher plants
    Somerville, C
  • Toward a systems approach to understanding plant cell walls
    Somerville, C; Bauer, S; Brininstool, G; Facette, M; Hamann, T; Milne, J; Osborne, E; Paredez, A; Persson, S; Raab, T; Vorwerk, S; Youngs, H
  • Overexpression of a novel Arabidopsis PP2C isoform, AtPP2CF1, enhances plant biomass production by increasing inflorescence stem growth
    Sugimoto, H; Kondo, S; Tanaka, T; Imamura, C; Muramoto, N; Hattori, E; Ogawa, K; Mitsukawa, N; Ohto, C
  • An Arabidopsis gene regulatory network for secondary cell wall synthesis
    Taylor-Teeples, M; Lin, L; Lucas, M; Turco, G; Toal, TW; Gaudinier, A; Young, NF; Trabucco, GM; Veling, MT; Lamothe, R; Handakumbura, PP; Xiong, G; Wang, C; Corwin, J; Tsoukalas, A; Zhang, L; Ware, D; Pauly, M; Kliebenstein, DJ; Dehesh, K; Tagkopoulos, I; Breton, G; Pruneda-Paz, JL; Ahnert, SE; Kay, SA; Hazen, SP; Brady, SM
  • Semimicro determination of cellulose in biological materials
    Updegraff, DM
  • PIECE 2.0: an update for the plant gene structure comparison and evolution database
    Wang, Y; Xu, L; Thilmony, R; You, FM; Gu, YQ; Coleman-Derr, D
  • Genome evolution in polyploids
    Wendel, JF
  • Recent advances in cotton genomics
    Zhang, HB; Li, YN; Wang, BH; Chee, PW
  • Secondary cell walls: biosynthesis, patterned deposition and transcriptional regulation
    Zhong, R; Ye, ZH
  • The MYB46 transcription factor is a direct target of SND1 and regulates secondary wall biosynthesis in Arabidopsis
    Zhong, R; Richardson, EA; Ye, ZH