Ectopic expression a tomato KNOX Gene Tkn4 affects the formation and the differentiation of meristems and vasculature

Ectopic expression a tomato KNOX Gene Tkn4 affects the formation and the differentiation of... The KNOTTED-LIKE HOMEODOMAIN genes are involved in maintenance of the shoot apical meristem which produces the whole above-ground body of vascular plants. In this report, a tomato homolog gene, named as Tkn4 (a nucleus targeted transcription factor) was identified and characterized. By performing RT-PCR, the transcript level of Tkn4 was separately found in stem, root, stamen, stigma, fruit and sepal but hardly visible in the leaf. Besides, Tkn4 was induced by a series of plant hormones. Overexpression of Tkn4 gene in tomato resulted in dwarf phenotype and strongly repressed the formation of shoot apical meristem, lateral meristem and cambiums in transgenic lines. The transgenic lines had wrinkled leaves and anatomic analysis showed that there was no obvious palisade tissues in the leaves and the layer of cells changed in vascular tissue (xylem and phloem). To explore the regulation network of Tkn4, RNA-sequencing was performed in overexpression lines and wild type plants, by which many genes related to the synthesis and the signal transduction of cytokinin, auxin, gibberellin, ethylene, abscisic acid, and tracheary element differentiation or extracellular matrix synthesis were significantly regulated. Taken together, our results demonstrate that Tkn4 plays important roles in regulating the biosynthesis and signal transduction of diverse plant hormones, and the formation and differentiation of meristems and vasculature in tomato. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Ectopic expression a tomato KNOX Gene Tkn4 affects the formation and the differentiation of meristems and vasculature

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Publisher
Springer Netherlands
Copyright
Copyright © 2015 by Springer Science+Business Media Dordrecht
Subject
Life Sciences; Plant Sciences; Biochemistry, general; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-015-0387-x
Publisher site
See Article on Publisher Site

Abstract

The KNOTTED-LIKE HOMEODOMAIN genes are involved in maintenance of the shoot apical meristem which produces the whole above-ground body of vascular plants. In this report, a tomato homolog gene, named as Tkn4 (a nucleus targeted transcription factor) was identified and characterized. By performing RT-PCR, the transcript level of Tkn4 was separately found in stem, root, stamen, stigma, fruit and sepal but hardly visible in the leaf. Besides, Tkn4 was induced by a series of plant hormones. Overexpression of Tkn4 gene in tomato resulted in dwarf phenotype and strongly repressed the formation of shoot apical meristem, lateral meristem and cambiums in transgenic lines. The transgenic lines had wrinkled leaves and anatomic analysis showed that there was no obvious palisade tissues in the leaves and the layer of cells changed in vascular tissue (xylem and phloem). To explore the regulation network of Tkn4, RNA-sequencing was performed in overexpression lines and wild type plants, by which many genes related to the synthesis and the signal transduction of cytokinin, auxin, gibberellin, ethylene, abscisic acid, and tracheary element differentiation or extracellular matrix synthesis were significantly regulated. Taken together, our results demonstrate that Tkn4 plays important roles in regulating the biosynthesis and signal transduction of diverse plant hormones, and the formation and differentiation of meristems and vasculature in tomato.

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 11, 2015

References

  • Differentiation of vascular tissues
    Aloni, R
  • The Arabidopsis ATHB-8 HD-zip protein acts as a differentiation-promoting transcription factor of the vascular meristems
    Baima, S; Possenti, M; Matteucci, A; Wisman, E; Altamura, MM; Ruberti, I; Morelli, G
  • KNAT6: an Arabidopsis homeobox gene involved in meristem activity and organ separation
    Belles-Boix, E; Hamant, O; Witiak, SM; Morin, H; Traas, J; Pautot, V
  • The maize transcription factor KNOTTED1 directly regulates the gibberellin catabolism gene ga2ox1
    Bolduc, N; Hake, S
  • Compound leaf development and evolution in the legumes
    Champagne, CEM; Golibera, TE; Wojciechowskib, MF; Meia, RW; Townsleya, BT; Wangc, K; Pazc, MM; Geetad, R; Sinhaa, N
  • A gene fusion at a homeobox locus: alterations in leaf shape and implications for morphological evolution
    Chen, J-J; Janssen, B-J; Williams, A; Sinha, N
  • Kinetics of determination in the differentiation of isolated mesophyll cells of Zinnia elegans to tracheary elements
    Church, DL; Galston, AW
  • Gibberellin dose-response regulation of GA4 gene transcript levels in Arabidopsis
    Cowling, RJ; Kamiya, Y; Seto, H; Harberd, NP
  • KNAT1 and ERECTA regulate inflorescence architecture in Arabidopsis
    Douglas, SJ; Chuck, G; Dengler, RE; Pelecanda, L; Riggs, CD

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