1067-4136/05/3601- © 2005 Pleiades Publishing, Inc.
Russian Journal of Ecology, Vol. 36, No. 1, 2005, pp. 48–51. Translated from Ekologiya, No. 1, 2005, pp. 55–58.
Original Russian Text Copyright © 2005 by Endonova, Antsupova.
The study of the inﬂuence of environmental condi-
tions on concentrations of biologically active sub-
stances in plants is important for understanding the
physiological processes occurring in plants.
The inﬂuence of natural climatic conditions on the
accumulation of alkaloids and ﬂavonoids in
in Transbaikalia has not yet
been studied, although these species are widely used in
traditional Tibetan, Mongolian, and popular medicine.
In Tibetan medicine, the inﬂorescences of
are used to treat inﬂammatory diseases of the lungs and
gastrointestinal tract, and the above-ground parts are
used in the case of sepsis. The above-ground parts of
are used in the case of gastric and gyne-
cologic diseases, fever, and tuberculosis. The inﬂores-
cences and roots are used against syphilis and pulmo-
nary tuberculosis, and they are used externally to elim-
inate warts, against scabies and rash, callus, snake
bites, etc. (
An analysis of published data showed that luteolin,
diosmetin, and chlorogenic, nonchlorogenic, and caf-
feic acids were extracted from the inﬂorescences of
(Rezanova and Naidakova, 1974). Cardiac
glycosides (Blinova and Stukkei, 1961), saponins,
traces of alkaloids (Kuvaev and Blinova, 1961), ﬂa-
vonoids (Bobrova, 1961), and coumarins (Dargaeva
and Brutko, 1977) were detected in the above-ground
plant parts using qualitative reactions. In
-D-glucopyranoside and querci-
meritrin (Zemtsova and Bandyukova, 1968) were iso-
lated, and ﬂavonoid ochrosid was found (Zemtsova
plants were col-
lected in 2001–2002 at the ﬂowering phase in different
regions of the Buryat Republic and Chita oblast. A total
of 48 plant samples were studied. Measurements were
made in ﬁve replications. To study the seasonal dynam-
ics or alkaloid and ﬂavonoid concentrations, the sam-
ples were taken at different phenological phases (begin-
ning of growing, budding, ﬂowering, and fruiting).
Environmental conditions in the natural ranges of
, in the forest-steppe and
steppe zones of southern Transbaikalia, differ in topog-
raphy, soils, and other factors.
The concentrations of alkaloids in both scabious
species were determined gravimetrically (Taizhanov
, 1972), and concentrations of ﬂavonoids, by the
conventional method (
The results showed that alkaloids and ﬂavonoids
were present in all organs of
, but their amounts depended on the place of
growth, and the plants accumulated greater amounts of
ﬂavonoids than alkaloids. The average concentrations
of total alkaloids in
varied from 0.1 to 0.9%
in inﬂorescences, from 0.1 to 0.8% in leaves and stems,
and from 0.05 to 0.33% in roots. The concentrations of
total ﬂavonoids were 2.03–5.1% in the inﬂorescences,
1.15–4.8% in leaves and stems, and 0.14–0.33% in
roots (Table 1). The concentrations of total alkaloids in
the inﬂorescences of
varied from 0.1 to
0.2%; in leaves and stems, from 0.15 to 0.26%; and in
roots, from 0.1 to 0.2%. The concentrations of total ﬂa-
vonoids were 4.2–6.0% in the inﬂorescences, 1.9–2.4%
in leaves and stems, and 0.14–0.15% in roots.
The highest total content of ﬂavonoids and alkaloids
was revealed in the inﬂorescences of
Its value in plant samples collected in
2002 was slightly higher than in 2001, probably
because the summer of 2002 was hotter and drier.
The concentration of total ﬂavonoids in
signiﬁcantly varied depending on habitat. For example,
the highest concentration of ﬂavonoids in inﬂores-
cences (5.1%) was detected in plants growing in an
open area on sandy soil, and the lowest concentration
was characteristic of plants from the southern slope of
a cone-shaped hill with chestnut soil (2.29%). The sam-
ples with the highest and lowest contents of alkaloids
(0.91 and 0.1%) were collected from gray forest soil
and chestnut soil, respectively.
Dynamics of Accumulation of Alkaloids and Flavonoids
Fisch. ex Roem. et Schult.
G. B. Endonova and T. P. Antsupova
East Siberian State Technological University,
ul. Klyuchevskaya 40a, Ulan-Ude, 670013 Buryat Republic, Russia
Received May 8, 2003
: habitat, content, dynamics, alkaloid, ﬂavonoid, growth,