Arch Microbiol (2017) 199:1065–1068
Draft genome sequence of the potato pathogen Rhizoctonia solani
AG3‑PT isolate Ben3
· Franziska Genzel
· Bart Verwaaijen
· Jochen Blom
· Oliver Rupp
· Rita Zrenner
· Rita Grosch
· Alfred Pühler
· Andreas Schlüter
Received: 9 March 2017 / Revised: 31 May 2017 / Accepted: 2 June 2017 / Published online: 8 June 2017
© Springer-Verlag GmbH Germany 2017
Keywords Ultrafast sequencing · AUGUSTUS ·
GenDBE · EDGAR
Rhizoctonia solani Kühn [anamorph, teleomorph Than-
atephorus cucumeris (Frank) Donk] is a soil-borne fungus
belonging to the phylum Basidiomycota. The fungus R.
solani was ﬁrst observed on diseased potato tubers in Ger-
many by Julius Kühn in 1858 (Ogoshi 1987). Diseases on
potato caused by R. solani occur in all areas where potatoes
are grown and affect the qualitative and quantitative yield
of potato tubers globally (Fiers et al. 2011; Lehtonen et al.
2008; Woodhall et al. 2007). Potato tuber yield losses of
approximately 30% have been observed (Banville 1989).
R. solani comprises a species complex of various genetic
groups called anastomosis groups (AG) (Carling et al.
2002). Hyphae of isolates belonging to the same AG are
able to anastomose. Currently, 13 AGs are known; their
members differ in morphological characteristics and host
ranges (Vilgalys and Cubeta 1994; Carling et al. 2002).
Members of different AGs, in ﬁrst instance, can also be dif-
ferentiated at the genetic level (Sharon et al. 2008). Rhizoc-
tonia solani AG3 was found to be the predominant AG
associated with potato and is referred to as R. solani AG3-
potato type (PT) (Campion et al. 2003; Fiers et al. 2011;
Woodhall et al. 2007; Lehtonen et al. 2008).
Another sub-group of R. solani AG3 is associated with
diseases of tobacco and therefore designated tobacco type
(R. solani AG3-TT, Kuninaga et al. 2000). Infection of
potato by R. solani AG3-PT may result in two distinguish-
able disease phenotypes, namely stem canker and black
scurf (Wilson et al. 2008). Early in the growth period,
stem canker can be recognized as necrotic lesions on
Abstract The basidiomycetes fungus Rhizoctonia solani
AG3 is responsible for black scurf disease on potato and
occurs in each potato growing area world-wide. In this
study, the draft genome sequence of the black scurf path-
ogen R. solani AG3-PT isolate Ben3 is presented. The
genome sequence of R. solani AG3-PT isolate Ben3 con-
sists of 1385 scaffolds. These scaffolds amount to a size
of approx. 51 Mb. Considering coverage analyses of con-
tigs, the size of the diploid genome was estimated to cor-
respond to 116 Mb. Gene prediction by applying AUGUS-
TUS (3.2.1.) resulted in 12,567 identiﬁed genes. Based on
automatic annotation using GenDBE, genes potentially
encoding cellulases and enzymes involved in secondary
metabolite synthesis were identiﬁed in the R. solani AG3-
PT isolate Ben3 genome. Comparative analyses including
the R. solani AG3 isolate Rhs1AP, also originating from
potato, revealed ﬁrst insights into core genes shared by
both isolates and unique determinants of each isolate.
Communicated by Erko Stackebrandt.
Electronic supplementary material The online version of this
article (doi:10.1007/s00203-017-1394-x) contains supplementary
material, which is available to authorized users.
* Daniel Wibberg
Genome Research of Industrial Microorganisms, CeBiTec,
Bielefeld University, 33501 Bielefeld, Germany
Department Plant Health, Leibniz Institute of Vegetable
and Ornamental Crops (IGZ), 14979 Großbeeren, Germany
Bioinformatics and Systems Biology, Gießen University,
35392 Gießen, Germany