Does MYC2 really play a negative role in jasmonic acid-induced indolic glucosinolate biosynthesis in Arabidopsis thaliana?

Does MYC2 really play a negative role in jasmonic acid-induced indolic glucosinolate biosynthesis... Glucosinolates are a group of sulfur-rich thioglucosides and are known to play an important role in plant defense and benefit to human health. The accumulation of indolic glucosinolates (IGS) is strongly induced by jasmonic acid (JA); however, the underlying molecular mechanism remains only partly understood. MYC3 and MYC4, the homologs of MYC2 have been shown to act additively with MYC2 in the activation of some JA responses. In this study, quantitative RT-PCR revealed that the expression of MYC3 and MYC4 was induced to a higher level in myc2 mutants compared with WT plants after 6 h of methyl jasmonate (MeJA) treatment. Likewise, the transcript of MYB34, a known positive regulator of IGS biosynthesis, also showed a remarkable increase at 6 h in MeJA-treated myc2 mutants relative to that in MeJA-treated WT plants. Then, accordingly, the expression of tryptophan biosynthetic genes (ASA1, TSA1, and TSB1) was identified to be up-regulated at 6 h, but the induction of IGS biosynthetic genes (CYP79B2, CYP79B3, and CYP83B1) showed a temporally delayed increase at 24 h. Ultimately, the total IGS was detected to be increased at 24 h after MeJA treatment in myc2 mutants. Taken together, the compensatory activation of MYC3 and MYC4 in MeJA-treated myc2 mutants might occur temporally associated with the concurrent increase in the IGS biosynthesis, providing a new insight in the role of MYC2 in JA-induced IGS biosynthesis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Does MYC2 really play a negative role in jasmonic acid-induced indolic glucosinolate biosynthesis in Arabidopsis thaliana?

Loading next page...
 
/lp/springer_journal/does-myc2-really-play-a-negative-role-in-jasmonic-acid-induced-indolic-0MLcVIHzam
Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2013 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443713010020
Publisher site
See Article on Publisher Site

Abstract

Glucosinolates are a group of sulfur-rich thioglucosides and are known to play an important role in plant defense and benefit to human health. The accumulation of indolic glucosinolates (IGS) is strongly induced by jasmonic acid (JA); however, the underlying molecular mechanism remains only partly understood. MYC3 and MYC4, the homologs of MYC2 have been shown to act additively with MYC2 in the activation of some JA responses. In this study, quantitative RT-PCR revealed that the expression of MYC3 and MYC4 was induced to a higher level in myc2 mutants compared with WT plants after 6 h of methyl jasmonate (MeJA) treatment. Likewise, the transcript of MYB34, a known positive regulator of IGS biosynthesis, also showed a remarkable increase at 6 h in MeJA-treated myc2 mutants relative to that in MeJA-treated WT plants. Then, accordingly, the expression of tryptophan biosynthetic genes (ASA1, TSA1, and TSB1) was identified to be up-regulated at 6 h, but the induction of IGS biosynthetic genes (CYP79B2, CYP79B3, and CYP83B1) showed a temporally delayed increase at 24 h. Ultimately, the total IGS was detected to be increased at 24 h after MeJA treatment in myc2 mutants. Taken together, the compensatory activation of MYC3 and MYC4 in MeJA-treated myc2 mutants might occur temporally associated with the concurrent increase in the IGS biosynthesis, providing a new insight in the role of MYC2 in JA-induced IGS biosynthesis.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Dec 28, 2012

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from
Google Scholar,
PubMed
Create lists to
organize your research
Export lists, citations
Read DeepDyve articles
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off