DNA Methylation Differences in Soybean Hybrids and Their Parental Lines

DNA Methylation Differences in Soybean Hybrids and Their Parental Lines To understand the methylation level and genetic delivery from parental line to their hybrid in soybean (Glycine max (L.) Merr.), four groups of parental lines and their hybrids were analyzed using methylation sensitive amplification polymorphism (MSAP). Comparisons of the different methylation distributions revealed. (1) The methylation level of the whole hybrid was lower than that of the corresponding parent lines, and the internal cytosine-methylation pattern was dominant. (2) In this study, comparing the total methylation levels in hybrids and the midparent values we assume that the larger differences in DNA methylation between parental lines lead to stronger heterosis. (3) Four demethylated and one hyper-methylated fragments were sequenced and located in the reference genome by blast analysis. These methylation changes could cause expression levels changes of a protein kinase, zinc finger protein and a mitochondrial transcript, which would affect a variety of metabolic pathways in soybean, providing an explanation for the observed heterosis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

DNA Methylation Differences in Soybean Hybrids and Their Parental Lines

7 pages
Read Article

Loading next page...
 
/lp/springer_journal/dna-methylation-differences-in-soybean-hybrids-and-their-parental-pekc0qTEqI
Publisher site
See Article on Publisher Site

Abstract

To understand the methylation level and genetic delivery from parental line to their hybrid in soybean (Glycine max (L.) Merr.), four groups of parental lines and their hybrids were analyzed using methylation sensitive amplification polymorphism (MSAP). Comparisons of the different methylation distributions revealed. (1) The methylation level of the whole hybrid was lower than that of the corresponding parent lines, and the internal cytosine-methylation pattern was dominant. (2) In this study, comparing the total methylation levels in hybrids and the midparent values we assume that the larger differences in DNA methylation between parental lines lead to stronger heterosis. (3) Four demethylated and one hyper-methylated fragments were sequenced and located in the reference genome by blast analysis. These methylation changes could cause expression levels changes of a protein kinase, zinc finger protein and a mitochondrial transcript, which would affect a variety of metabolic pathways in soybean, providing an explanation for the observed heterosis.

Journal

Russian Journal of Plant Physiology – Springer Journals

Published: Jun 1, 2018

References

  • Epigenetic inheritance and variation of DNA methylation level and pattern in maize intra-specific hybrids
    Zhao, X.; Chai, Y.; Liu, B.
  • Alterations in cytosine methylation and species-specific transcription induced by interspecific hybridization between Oryza sativa and O. officinalis
    Jin, H.; Hu, W.; Wei, Z.; Wan, L.; Li, G.; Tan, G.; Zhu, L.; He, G.
  • Intraspecific hybrids of Arabidopsis thaliana revealed no gross alterations in endopolyploidy, DNA methylation, histone modifications and transcript levels
    Banaei Moghaddam, A.M.; Fuchs, J.; Czauderna, T.; Houben, A.; Mette, M.F.
  • Mid-century controversies in population genetics
    Crow, J.F.
  • The genetics of inbreeding depression
    Charlesworth, D.; Willis, J.H.
  • Alterations in inheritance pattern and level of cytosine DNA methylation, and their relationship with heterosis in rice
    Sakthivel, K.; Girishkumar, K.; Ramkumar, G.; Shenoy, V.; Kajjidoni, S.; Salimath, P.
  • Genome-wide analysis of DNA methylation and gene expression changes in two Arabidopsis ecotypes and their reciprocal hybrids
    Shen, H.; He, H.; Li, J.; Chen, W.; Wang, X.; Guo, L.; Peng, Z.; He, G.; Zhong, S.; Qi, Y.; Terzaghi, W.; Deng, X.W.
  • DNA methylation status is associated with the formation of heterosis in Larix kaempferi intraspecific hybrids
    Li, A.; Song, W.Q.; Chen, C.B.; Zhou, Y.N.; Qi, L.W.; Wang, C.G.
  • Plant Breeding Reviews
    Palmer, R.G.; Gai, J.; Sun, H.; Burton, J.W.
  • Application of SSR markers for purity testing of commercial hybrid soybean (Glycine max L.)
    Zhang, C.B.; Peng, B.; Zhang, W.L.; Wang, S.M.; Sun, H.; Dong, Y.S.; Zhao, L.M.
  • Modification of a CTAB DNA extraction protocol for plants containing high polysaccharides and polyphenol component
    Porebski, S.; Bailey, L.G.; Baum, B.R.
  • AFLP: a new technique for DNA fingerprinting
    Vos, P.; Hogers, R.; Bleeker, M. v d; Lee, T.; Hornes, M.; Frijters, A.; Pot, J.; Peleman, J.; Kuiper, M.; Zabeau, M.
  • Brassica oleracea displays a high level of DNA methylation polymorphism
    Salmon, A.; Clotault, J.; Jenczewski, E.; Chable, V.; Manzanares-Dauleux, M.J.
  • DNA methylation polymorphism in annual wild soybean (Glycine soja Sieb. et Zucc.) and cultivated soybean (G. max L. Merr.)
    Zhong, X.; Wang, Y.; Liu, X.; Gong, L.; Ma, Y.; Qi, B.; Dong, Y.S.; Liu, B.
  • Genomewide analysis of DNA methylation in soybean
    Song, Q.; Lu, X.; Li, Q.; Chen, H.; Hu, X.; Ma, B.; Zhang, W.K.; Chen, S.Y.; Zhang, J.S.
  • How to interpret methylation sensitive amplified polymorphism (MSAP) profiles
    Fulneček, J.; Kovařík, A.
  • Genome-wide high-resolution mapping and functional analysis of DNA methylation in Arabidopsis
    Zhang, X.Y.; Yazaki, J.; Sundaresan, A.; Cokus, S.; Chan, S.W.; Chen, H.M.; Henderson, I.R.; Shinn, P.; Pellegrini, M.; Jacobsen, S.E.; Ecker, J.R.
  • Genome-wide analysis of Arabidopsis thaliana DNA methylation uncovers an interdependence between methylation and transcription
    Zilberman, D.; Gehring, M.; Tran, R.K.; Ballinger, T.; Henikoff, S.
  • Patterns of cytosine methylation in an elite rice hybrid and its parental lines, detected by a methylation-sensitive amplification polymorphism technique
    Xiong, L.; Xu, C.; Maroof, M.S.; Zhang, Q.
  • Analysis of DNA methylation in cotton hybrids and their parents
    Zhao, Y.; Yu, S.; Xing, C.; Fan, S.; Song, M.
  • Gene body methylation is conserved between plant orthologs and is of evolutionary consequence
    Takuno, S.; Gaut, B.S.
  • Gene body methylation shows distinct patterns associated with different gene origins and duplication modes and has a heterogeneous relationship with gene expression in Oryza sativa (rice)
    Wang, Y.P.; Wang, X.Y.; Lee, T.H.; Mansoor, S.; Paterson, A.H.

You’re reading a free preview. Subscribe to read the entire article.

Try 2 weeks free now

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

or browse the journals available

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off

Sign up
for free
Start 14 day
Free Trial