99m Objectives To investigate DNA double-strand breaks (DSBs) in blood lymphocytes induced by two-day Tc-MIBI myocardial 99m perfusion scintigraphy (MPS) using y-H2AX immunofluorescence microscopy and to correlate the results with Tc activity in blood samples. Methods Eleven patients who underwent two-day MPS were included. DSB blood sampling was performed before and 5min, 1h 99m and 24h after the first and second radiotracer injections. Tc activity was measured in each blood sample. For immunofluo- rescence microscopy, distinct foci representing DSBs were quantified in lymphocytes after staining for the phosphorylated histone variant y-H2AX. 99m Results The Tc-MIBI activity measured on days one and two was similar (254±25 and 258±27 MBq; p=0.594). Compared with baseline DSB foci (0.09±0.05/cell), a significant increase was found at 5min (0.19±0.04/cell) and 1h (0.18±0.04/cell) after the first injection and at 5min and 1h after the second injection (0.21±0.03 and 0.19±0.04/cell, respectively; p=0.003 for both). At 24h after the first and second injections, the number of DSB foci had returned to 99m baseline (0.06±0.02 and 0.12±0.05/cell, respectively). Tc activity levels in peripheral blood samples correlated well with DSB counts (r=0.451). 99m Conclusions DSB counts reflect Tc-MIBI activity after injection for two-day MPS, and might allow individual monitoring of
European Radiology – Springer Journals
Published: Jan 30, 2018
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