DNA double-strand breaks in blood lymphocytes induced by two-day 99mTc-MIBI myocardial perfusion scintigraphy

DNA double-strand breaks in blood lymphocytes induced by two-day 99mTc-MIBI myocardial perfusion... 99m Objectives To investigate DNA double-strand breaks (DSBs) in blood lymphocytes induced by two-day Tc-MIBI myocardial 99m perfusion scintigraphy (MPS) using y-H2AX immunofluorescence microscopy and to correlate the results with Tc activity in blood samples. Methods Eleven patients who underwent two-day MPS were included. DSB blood sampling was performed before and 5min, 1h 99m and 24h after the first and second radiotracer injections. Tc activity was measured in each blood sample. For immunofluo- rescence microscopy, distinct foci representing DSBs were quantified in lymphocytes after staining for the phosphorylated histone variant y-H2AX. 99m Results The Tc-MIBI activity measured on days one and two was similar (254±25 and 258±27 MBq; p=0.594). Compared with baseline DSB foci (0.09±0.05/cell), a significant increase was found at 5min (0.19±0.04/cell) and 1h (0.18±0.04/cell) after the first injection and at 5min and 1h after the second injection (0.21±0.03 and 0.19±0.04/cell, respectively; p=0.003 for both). At 24h after the first and second injections, the number of DSB foci had returned to 99m baseline (0.06±0.02 and 0.12±0.05/cell, respectively). Tc activity levels in peripheral blood samples correlated well with DSB counts (r=0.451). 99m Conclusions DSB counts reflect Tc-MIBI activity after injection for two-day MPS, and might allow individual monitoring of http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Radiology Springer Journals

DNA double-strand breaks in blood lymphocytes induced by two-day 99mTc-MIBI myocardial perfusion scintigraphy

7 pages
Read Article
Loading next page...
 
/lp/springer_journal/dna-double-strand-breaks-in-blood-lymphocytes-induced-by-two-day-99mtc-455yjrY49x
Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2018 by European Society of Radiology
Subject
Medicine & Public Health; Imaging / Radiology; Diagnostic Radiology; Interventional Radiology; Neuroradiology; Ultrasound; Internal Medicine
ISSN
0938-7994
eISSN
1432-1084
D.O.I.
10.1007/s00330-017-5239-4
Publisher site
See Article on Publisher Site

Abstract

99m Objectives To investigate DNA double-strand breaks (DSBs) in blood lymphocytes induced by two-day Tc-MIBI myocardial 99m perfusion scintigraphy (MPS) using y-H2AX immunofluorescence microscopy and to correlate the results with Tc activity in blood samples. Methods Eleven patients who underwent two-day MPS were included. DSB blood sampling was performed before and 5min, 1h 99m and 24h after the first and second radiotracer injections. Tc activity was measured in each blood sample. For immunofluo- rescence microscopy, distinct foci representing DSBs were quantified in lymphocytes after staining for the phosphorylated histone variant y-H2AX. 99m Results The Tc-MIBI activity measured on days one and two was similar (254±25 and 258±27 MBq; p=0.594). Compared with baseline DSB foci (0.09±0.05/cell), a significant increase was found at 5min (0.19±0.04/cell) and 1h (0.18±0.04/cell) after the first injection and at 5min and 1h after the second injection (0.21±0.03 and 0.19±0.04/cell, respectively; p=0.003 for both). At 24h after the first and second injections, the number of DSB foci had returned to 99m baseline (0.06±0.02 and 0.12±0.05/cell, respectively). Tc activity levels in peripheral blood samples correlated well with DSB counts (r=0.451). 99m Conclusions DSB counts reflect Tc-MIBI activity after injection for two-day MPS, and might allow individual monitoring of

Journal

European Radiology – Springer Journals

Published: Jan 30, 2018

References

  • Exposure to low-dose ionizing radiation from medical imaging procedures
    Fazel, R; Krumholz, HM; Wang, Y
  • Risks and benefits of cardiac imaging: an analysis of risks related to imaging for coronary artery disease
    Knuuti, J; Bengel, F; Bax, JJ
  • Radiation exposure from imaging tests: is there an increased cancer risk?
    Nguyen, PK; JC, W
  • gamma-H2AX foci as a biomarker for patient X-ray exposure in pediatric cardiac catheterization: are we underestimating radiation risks?
    Beels, L; Bacher, K; Wolf, D; Werbrouck, J; Thierens, H
  • Leukocyte DNA damage after multi-detector row CT: a quantitative biomarker of low-level radiation exposure
    Rothkamm, K; Balroop, S; Shekhdar, J; Fernie, P; Goh, V
  • In vivo formation of gamma-H2AX and 53BP1 DNA repair foci in blood cells after radioiodine therapy of differentiated thyroid cancer
    Lassmann, M; Hanscheid, H; Gassen, D
  • gamma-H2AX foci as in vivo effect biomarker in children emphasize the importance to minimize x-ray doses in paediatric CT imaging
    Vandevoorde, C; Franck, C; Bacher, K
  • Use of diagnostic imaging studies and associated radiation exposure for patients enrolled in large integrated health care systems, 1996-2010
    Smith-Bindman, R; Miglioretti, DL; Johnson, E
  • Assessing the need for nuclear cardiology and other advanced cardiac imaging modalities in the developing world
    Vitola, JV; Shaw, LJ; Allam, AH
  • EANM procedural guidelines for radionuclide myocardial perfusion imaging with SPECT and SPECT/CT: 2015 revision
    Verberne, HJ; Acampa, W; Anagnostopoulos, C
  • Computed tomography angiography and perfusion to assess coronary artery stenosis causing perfusion defects by single photon emission computed tomography: the CORE320 study
    Rochitte, CE; George, RT; Chen, MY
  • EANM/ESC procedural guidelines for myocardial perfusion imaging in nuclear cardiology
    Hesse, B; Tagil, K; Cuocolo, A
  • DNA double-strand breaks as potential indicators for the biological effects of ionising radiation exposure from cardiac CT and conventional coronary angiography: a randomised, controlled study
    Geisel, D; Zimmermann, E; Rief, M
  • The influence of x-ray contrast agents in computed tomography on the induction of dicentrics and gamma-H2AX foci in lymphocytes of human blood samples
    Jost, G; Golfier, S; Pietsch, H
  • Flow cytometric analysis of phosphorylated histone H2AX following exposure to ionizing radiation in human microvascular endothelial cells
    Kataoka, Y; Bindokas, VP; Duggan, RC; Murley, JS; Grdina, DJ
  • Direct and Auger Electron-Induced, Single- and Double-Strand Breaks on Plasmid DNA Caused by 99mTc-Labeled Pyrene Derivatives and the Effect of Bonding Distance
    Reissig, F; Mamat, C; Steinbach, J
  • Effect of CT scan protocols on x-ray-induced DNA double-strand breaks in blood lymphocytes of patients undergoing coronary CT angiography
    Kuefner, MA; Grudzenski, S; Hamann, J
  • X-ray induced DNA double-strand breaks in coronary CT angiography: comparison of sequential, low-pitch helical and high-pitch helical data acquisition
    Brand, M; Sommer, M; Achenbach, S
  • Unjustified CT examinations in young patients
    Oikarinen, H; Merilainen, S; Paakko, E; Karttunen, A; Nieminen, MT; Tervonen, O
  • Image quality and dose optimisation for infant CT using a paediatric phantom
    Lambert, JW; Phelps, AS; Courtier, JL; Gould, RG; MacKenzie, JD
  • Optimizing radiation dose by using advanced modelled iterative reconstruction in high-pitch coronary CT angiography
    Gordic, S; Desbiolles, L; Sedlmair, M
  • Induction and repair of DNA double-strand breaks in blood lymphocytes of patients undergoing (1)(8)F-FDG PET/CT examinations
    May, MS; Brand, M; Wuest, W
  • Dose-length product of scanners correlates with DNA damage in patients undergoing contrast CT
    Beels, L; Bacher, K; Smeets, P; Verstraete, K; Vral, A; Thierens, H
  • Hybrid cardiac imaging: SPECT/CT and PET/CT. A joint position statement by the European Association of Nuclear Medicine (EANM), the European Society of Cardiac Radiology (ESCR) and the European Council of Nuclear Cardiology (ECNC)
    Flotats, A; Knuuti, J; Gutberlet, M
  • Technetium-99m hexakis 2-methoxyisobutyl isonitrile: human biodistribution, dosimetry, safety, and preliminary comparison to thallium-201 for myocardial perfusion imaging
    Wackers, FJ; Berman, DS; Maddahi, J
  • gammaH2AX as a marker of DNA double strand breaks and genomic instability in human population studies
    Valdiglesias, V; Giunta, S; Fenech, M; Neri, M; Bonassi, S
  • DNA damage in lymphocytes induced by cardiac CT and comparison with physical exposure parameters
    Fukumoto, W; Ishida, M; Sakai, C
  • Variable activation of the DNA damage response pathways in patients undergoing single-photon emission computed tomography myocardial perfusion imaging
    Lee, WH; Nguyen, P; Hu, S
  • An optimized method for measurement of gamma-H2AX in blood mononuclear and cultured cells
    Muslimovic, A; Ismail, IH; Gao, Y; Hammarsten, O
  • Experimental Sarcocystis suicanis infections: disease in growing pigs
    Barrows, PL; Prestwood, AK; Green, CE
  • 8-Oxo-2'-deoxyguanosine level in lymphocytes DNA of cancer patients undergoing radiotherapy
    Bialkowski, K; Kowara, R; Windorbska, W; Olinski, R

You’re reading a free preview. Subscribe to read the entire article.

Try 2 weeks free now

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

or browse the journals available

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off

Sign up
for free
Start 14 day
Free Trial