DNA Composition and Mitotic Time Cycle Duration
in the Evolution of Faba Bean and Its Wild Relatives
Ajay Kumar Parida
Soom Nath Raina
Received: 5 April 2016 / Accepted: 10 May 2016 / Published online: 28 May 2016
Ó The National Academy of Sciences, India 2016
Abstract The composition of nuclear DNA and mitotic
time cycle in Vicia faba, commonly known as Faba bean,
and its close relatives grouped together in Narbonensis
species complex are compared. The melting proﬁle and
buoyant density gradient analysis in the species, V. nar-
bonensis, V. serratifolia, V. galilaea and V. hyaeniscyamus
belonging to Narbonensis species complex and V. faba did
not show any appreciable change in the base composition.
The density gradient proﬁles in the species belonging to the
complex showed the presence of a distinct satellite fraction
in each species. This satellite fraction was absent in V.
faba. The change in the total duration or the duration of
different stages of mitotic cycle in the species belonging to
the species complex was not signiﬁcantly different. In
comparison, V. faba had longer mitotic cycle duration.
Keywords Broad bean Á DNA composition Á
Mitotic time cycle Á Wild relatives
Among the 140 Vicia species , it is V. faba, commonly
known as Faba bean, and its close wild relatives, and the taxa
within Sativa species complex, commonly known as vetchs,
are attracting considerable attention. The faba bean is one of
the most important grain legumes. The high protein content
of seeds, the ability to improve soil quality, and the resistance
to weeds after sowing, are some of the characteristic features
which are promoting interest in the crop. Successful efforts
have been made to genetically improve the species for higher
yield, bigger pods and seeds, and for better adaptability to
various geographical regions. Most of the cultivars, how-
ever, are still highly susceptible to a few pathogens and pests
and lack frost tolerance.
There are several closely related wild or semicultivated
species which have been placed alongwith V. faba and V.
bithynica in section Faba. Most of the authors have
grouped these species together to form Narbonensis species
complex. This complex contains V. narbonensis, V. ser-
ratifolia, V. galilaea, V. johannis, V. hyaeniscyamus, V.
kalakhensis and V. eristaliodes [2, 3].
All taxa of this complex show close morphological
resemblance with V. faba. It has been suggested that any one,
or more, species within the Narbonensis species complex
could be wild progenitors of V. faba [2, 4–9]. Several authors
[10–20] however, contradict this conclusion. The present
study is concerned with a few components of genome anal-
ysis of the species belonging to Narbonensis species complex
and V. faba. Its purpose is to offer new aspects, and to allow
an insight into the extent and nature of genomic analysis
ﬁrstly within the complex and secondly between these and V.
faba, which is of paramount importance for the determina-
tion of sources of germplasm for intelligent breeding and
other related programmes.
& Soom Nath Raina
Amity Institute of Biotechnology, Amity University Sec 125,
Noida 201313, U.P., India
M. S. Swaminathan Research Foundation, 3rd Cross Street,
Institutional Area Taramani, Chennai, Tamilnadu, India
Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. (Jan–Mar 2018) 88(1):185–190