Analysis of complete genomes of thermophilic bacteria of the genus Geobacillus, oxidizing n-alkanes and crude oil revealed the ladA gene and its homologues. In the genomes of some strains, the ladA gene was not detected, although they were capable of growth on n-alkanes. Cloning with degenerate primers has previously revealed eight homologues of the alkB gene (alkB-geo1–alkB-geo8) encoding alkane hydroxylases in Geobacillus. In the present work, investigation of the alkB genes of several new strains of thermophilic, hydrocarbon-oxidizing bacilli was carried out. In the clone libraries obtained using degenerate primers for the alkB genes from five strains of the genera Geobacillus, Parageobacillus, and Aeribacillus, mostly the universal homologous genes alkB-geo1 and alkB-geo4 were revealed. Additional PCR amplification with specific primers for each of the eight known alkB homologues revealed the universal homologous genes only in some of the studied strains. A correlation was found between the set of the alkB-geo3–alkB-geo6 homologus genes from Geobacillus subterraneus strain K amplified with specific primers and the previously revealed a set of these homologous genes transcripted at different cultivation conditions. This correlation may be due to accumulation of the copies of individual homologues under different cultivation conditions, which results in higher sensitivity of specific primers. The least common homologue, alkB-geo7, which was not detected in the relevant clone libraries, was found in two strains, indicating the possibility of development of more specific primers for amplification of this homologue in order to reveal hydrocarbon-oxidizing bacteria of related genera Geobacillus–Parageobacillus in molecular ecological research.
Microbiology – Springer Journals
Published: Jun 2, 2018
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera