Biphenyl 2,3-dioxygenase is the key enzyme involved in the bacterial destruction of biphenyl and polychlorinated biphenyls (PCBs), which are highly stable toxic compounds. The diversity of bphA1 genes encoding the biphenyl 2,3-dioxygenase a-subunit of biphenyl-degrading bacteria from the microbial community of the Bering Sea coastal sediments (the Anadyr port area) was studied. The enrichment culture was obtained by the incubation of bottom sediments samples with biphenyl as the only carbon source. It was followed by total DNA extraction and PCR analysis with degenerate primers specific to the bacterial biphenyl 2,3-dioxygenase α-subunit genes. Subsequent cloning of the PCR products led to the identification of three types of aromatic dioxygenase genes, which appeared to be phylogenetically close to the genes of the biphenyl/toluene dioxygenase and 3-phenylpropionate dioxygenase subfamilies of the Actinomycetales bacteria.
Russian Journal of Genetics – Springer Journals
Published: Jul 25, 2015
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera