1063-0740/05/3102- © 2005 Pleiades Publishing, Inc.
Russian Journal of Marine Biology, Vol. 31, No. 2, 2005, pp. 65–72.
Original Russian Text Copyright © 2005 by Biologiya Morya, Ivanova, Gorshkova, Mikhailov, Sergeev, Gladkikh, Goryachev, Dudarev, Botsul, Mozherovsky, Slinko, Kiselev.
The study of microbial communities in zones of
radioactive contamination and the role of microorgan-
isms in their evolution acquired particular importance
after the Chernobyl accident [6–8, 10]. As a result of
recent investigations of soil microbiota, approaches to
techniques for land decontamination have been gener-
ated . However, the role of microorganisms in bio-
geochemical cycles of radionuclides in the marine envi-
ronment has been studied inadequately. There is no
information the about numbers, taxonomic composi-
tion, or bioindication and degradation potential of
microorganisms in radioactive contamination areas on
the northwestern shelf of the Sea of Japan, particularly
Chazhma Bay where a nuclear accident on an atomic
submarine occurred 8.5 months before the Chernobyl
accident . This paper examines the distribution of
saprophytic bacteria in the water column of Chazhma
Bay and is the ﬁrst step to bridge up this gap.
MATERIALS AND METHODS
The materials were strains of marine microorgan-
isms isolated in October 2000 from water in the epicen-
ter of a nuclear accident on an atomic submarine (AS)
in Chazhma Bay in Peter the Great Bay (Fig. 1).
The total numbers of saprophytic microorganisms
were determined by direct inoculation (in three repli-
cates) of different volumes of water (30 and 100
diluted 10-fold in physiological solution in an agar-
enriched medium. Colony-forming units were enumer-
ated. The error was 10–15%.
The isolated colonies were incubated for 7 days at
C in a B agar-enriched medium (g/liter): peptone,
5.0; yeast extract, 2.0; glucose, 1.0; K
, 0.05; agar-agar, 20.0; distilled water, 500 ml;
Distribution of Saprophytic Bacteria in the Atomic Submarine
Accident Zone in Chazhma Bay, Sea of Japan
E. P. Ivanova
, N. M. Gorshkova
, V. V. Mikhailov
, A. F. Sergeev
R. V. Gladkikh
, V. A. Goryachev
, O. V. Dudarev
, A. I. Botsul
A. V. Mozherovsky
, E. N. Slinko
, and V. I. Kiselev
Paciﬁc Institute of Bioorganic Chemistry, Far East Division, Russian Academy of Sciences,
Vladivostok, 690022 Russia
Paciﬁc Oceanological Institute,
Vladivostok, 690041 Russia
Far Eastern Geological Institute,
Vladivostok, 690022 Russia
Received September 17, 2004
—The numerical and taxonomic composition of saprophytic bacteria was studied in the atomic sub-
marine accident zone in Chazhma Bay. With increasing depth in the epicenter of the accident zone, the mean
of the ratio of bacterial taxa in the resistance series tends toward a constant. The numerical structure of the
pelagic saprophytic bacterial assemblage becomes ordered as the bottom is approached. Analysis of radioactive
suspended matter allowed us to obtain model distribution of numbers of all saprophytes and each taxon from
the different environmental sources (sediment surface and upper layers of the sea).
Saprophytic bacteria, radioactive contamination, nuclear accident, Co-60, heavy metals.
Peter the Great Bay
Location of the study area in Peter the Great Bay.