1022-7954/02/3807- $27.00 © 2002
Russian Journal of Genetics, Vol. 38, No. 7, 2002, pp. 843–846. Translated from Genetika, Vol. 38, No. 7, 2002, pp. 1001–1005.
Original Russian Text Copyright © 2002 by Nersisyan, Martirosyan, Musaelyan, Meliksetyan.
This article presents a part of our medical genetic
study of various isoserologic systems in Nagornyi
Karabakh (NK) Armenians. Previously, we determined
frequencies of genes for erythrocyte systems AB0, Rh–
Hr, MNSs, Pp, Kidd, Duffy, Kell–Chellano, and Lewis
The HLA system is very informative for population
genetics because it is widely polymorphic and includes
tightly linked loci, whose antigens are a key link in the
immune response [3–5].
Population analysis in various regions of the world
provides grounds for investigation of the linkage
between HLA antigens and various disorders and adds
to the development of transfusion and transplantation
This paper presents the ﬁrst report on investigation
of 31 HLA antigens and genes of class I (A, B, and Cw)
and class II (DR) in NK Armenians.
We compared the immunogenetic indices of NK
Armenians obtained in this study with corresponding
data on the Armenian population of Armenia [9–12]
and major ethnic groups of the world [18, 19].
Venous blood samples were taken from 389 unre-
lated healthy Armenian men and women aged 19 to
50 years having at least four generations of ancestors
living in NK.
In 169 Armenians, we analyzed 7 antigens of the
(A1, A2, A3, A9, A10, A11, and A28) and
14 antigens of the locus
(B5, B7, B12, B13,
B14, B15, B16, B17, B18, B22, B27, B35, and B40). In
164 out of the 169 persons, we also analyzed four anti-
gens of the locus
(Cw1, Cw2, Cw4, and Cw5).
In 220 persons, six antigens of the locus
DR2, DR3, DR4, DR7, and DR10) were investigated.
Antigens of the loci
identiﬁed in the total population of lymphocytes by the
microlymphocytotoxical text. Lymphocytes were iso-
lated in a Ficoll–Verographin density gradient. Anti-
gens of the locus
in the B-population of lym-
phocytes were identiﬁed by the prolonged microlym-
phocytotoxical text. Populations of B lymphocytes
were isolated from peripheral blood on columns with
Nylon ﬁber .
Antigens of the loci A, B, and Cw were determined
with the use of an antiserum panel received from
Biotest-Serum and Institute of Hematology and Trans-
fusion, St.-Petersburg. Antigens of the locus DR were
investigated with monospeciﬁc DR antisera (Biotest-
Serum). Two to ﬁve antisera were used for determining
each antigen of the HLA system. Gene frequencies
were calculated by equation
= 1 –
the frequency of a corresponding antigen.
Genetic distances were determined by equation
is the genetic dis-
tance between populations 1 and 2,
frequencies in the populations, and
is the number of
allele pairs under comparison.
Tissue typing results were statistically processed as
The frequency distribution of antigens and genes of
HLA-A, B, Cw
in the population under
study is presented in Table 1.
Our study shows that all HLA antigens studied
occur in NK Armenians at different frequencies.
Distribution of HLA Antigens
in the Armenian Population
of Nagornyi Karabakh
V. M. Nersisyan, I. G. Martirosyan, N. O. Musaelyan, and N. O. Meliksetyan
Eolyan Hematological Center, Yerevan, 375014 Armenia
Received November 3, 2000; in ﬁnal form, October 1, 2001
—Characteristics of the distribution of 31 HLA antigens of classes I (A, B, and Cw) and II (DR) in
Nagornyi Karabakh Armenians are reported for the ﬁrst time. It has been found that the antigens most common
in this population are A2, A3, A9, B5, B7, B12, Cw4, DR4, DR2, and DR3; the least common antigens are B15,
B16, and B40. The results are compared with the data for Armenians living in Armenia and those for major
ethnic groups. The frequencies of HLA antigens in Nagornyi Karabakh Armenians match those in Armenians
living in Armenia. In the HLA-antigen distribution, Armenians are generally close to Caucasoids.