Distribution and characteristics of bovine leukemia virus integration sites in the host genome at three different clinical stages of infection

Distribution and characteristics of bovine leukemia virus integration sites in the host genome at... Bovine leukemia virus (BLV) is an oncogenic retrovirus closely related to human T-cell lymphotropic virus. BLV-infected cattle are categorized as asymptomatic carriers or as having persistent lymphocytosis or enzootic bovine leukemia, depending on the clinical stage. We investigated the BLV integration site distribution at three BLV clinical stages and examined genome sequence features around the integration sites. In all, 264 BLV integration sites, at various locations on each chromosome, were identified in 28 cattle by inverse PCR and BLAST searches. Approximately one-third of BLV proviruses were independently integrated within transcriptional units, and approximately 10 % were integrated near transcription start sites. Moreover, less than 7 % of BLV integration sites were located near CpG islands. BLV did not preferentially integrate into transcriptionally active regions during any of the clinical stages. At the nucleotide level, regions around BLV integration points were significantly A/T rich with weak sequence consensus. BLV preferentially integrated within long interspersed nuclear repeat elements. Although BLV integration sites may not be associated with disease progression, integration is selective at the nucleotide level. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Distribution and characteristics of bovine leukemia virus integration sites in the host genome at three different clinical stages of infection

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Publisher
Springer Vienna
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-014-2224-y
Publisher site
See Article on Publisher Site

References

  • Distribution and superinfection of bovine leukemia virus genotypes in Japan
    Asfaw, Y; Tsuduku, S; Konishi, M; Murakami, K; Tsuboi, T; Wu, D; Sentsui, H
  • Bovine leukemia virus specific antibodies among French cattle. I. Comparison of complement fixation and hematological tests
    Levy, D; Deshayes, L; Guillemain, B; Parodi, AL
  • Bovine leukemia virus integration site selection in cattle that develop leukemia
    Murakami, H; Yamada, T; Suzuki, M; Nakahara, Y; Suzuki, K; Sentsui, H
  • The recent prevalence of bovine leukemia virus (BLV) infection among Japanese cattle
    Murakami, K; Kobayashi, S; Konishi, M; Kameyama, K; Yamamoto, T; Tsutsui, T

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