Distinct Ca2+-permeable Cation Currents Are Activated by Internal Ca2+-Store Depletion in RBL-2H3 Cells and Human Salivary Gland Cells, HSG and HSY

Distinct Ca2+-permeable Cation Currents Are Activated by Internal Ca2+-Store Depletion in RBL-2H3... Store-operated Ca2+ influx, suggested to be mediated via store-operated cation channel (SOC), is present in all cells. The molecular basis of SOC, and possible heterogeneity of these channels, are still a matter of controversy. Here we have compared the properties of SOC currents (I SOC) in human submandibular glands cells (HSG) and human parotid gland cells (HSY) with I CRAC (Ca2+ release-activated Ca2+ current) in RBL cells. Internal Ca2+ store-depletion with IP3 or thapsigargin activated cation channels in all three cell types. 1 μM Gd3+ blocked channel activity in all cells. Washout of Gd3+ induced partial recovery in HSY and HSG but not RBL cells. 2-APB reversibly inhibited the channels in all cells. I CRAC in RBL cells displayed strong inward rectification with E rev(Ca) = >+90 mV and E rev (Na) = +60 mV. I SOC in HSG cells showed weaker rectification with E rev(Ca) = +25 mV and E rev(Na) = +10 mV. HSY cells displayed a linear current with E rev = +5 mV, which was similar in Ca2+- or Na+-containing medium. pCa/pNa was >500, 40, and 4.6 while pCs /pNa was 0.1,1, and 1.3, for RBL, HSG, and HSY cells, respectively. Evidence for anomalous mole fraction behavior of Ca2+/Na+ permeation was obtained with RBL and HSG cells but not HSY cells. Additionally, channel inactivation with Ca2+ + Na+ or Na+ in the bath was different in the three cell types. In aggregate, these data demonstrate that distinct store-dependent cation currents are stimulated in RBL, HSG, and HSY cells. Importantly, these data suggest a molecular heterogeneity, and possibly cell-specific differences in the function, of these channels. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Distinct Ca2+-permeable Cation Currents Are Activated by Internal Ca2+-Store Depletion in RBL-2H3 Cells and Human Salivary Gland Cells, HSG and HSY

Loading next page...
 
/lp/springer_journal/distinct-ca2-permeable-cation-currents-are-activated-by-internal-ca2-i3boBQMZ6y
Publisher
Springer-Verlag
Copyright
Copyright © 2004 by Springer Science+Business Media, Inc.
Subject
Life Sciences; Human Physiology; Biochemistry, general
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s00232-004-0698-3
Publisher site
See Article on Publisher Site

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial