Dissecting the transcriptional networks underlying the gibberellin response in Nicotiana tabacum

Dissecting the transcriptional networks underlying the gibberellin response in Nicotiana tabacum The present work examines global transcriptional and limited metabolic profiling to dissect the signalling pathways associated with gibberellin A (GA ) induced alterations in tobacco (Nicotiana tabacum L.) under field conditions. Our 3 3 microarray analysis revealed that GA foliar application elicited 7 032 differentially expressed genes (DEGs) with 3 507 and 3 525 genes displaying up- and down-regulation, respectively. These DEGs broadly belong to diverse metabolic pathways: GA signalling and homeostasis, ethylene and auxin biosyntheses, saccharide metabolism, glycolysis- tricarboxylic acid cycle, terpenoid biosynthesis, shikimate-phenylpropanoid pathway, S-adenosyl methionine cycle, fatty acid biosynthesis, cell wall metabolism, oxidative stress, DNA repair, and stress responses. Transcription validation of representative DEGs was confirmed by real-time quantitative PCR. A relative enhancement in cellular acetyl-CoA pool corroborated with a concomitant increase and decrease in transcriptions as well as activities of histone acetyltransferases and histone deacetylases, respectively. Phytochemical analysis shows an enhanced content of total sugars, reducing sugars, total flavonoids, and phenolics with a concomitant decrease of the content of starch, chlorophylls a and b, and carotenoids. Gas chromatography with mass spectrometry analysis revealed the presence of 30 differential metabolites, mostly belonging to terpenoid biosynthesis pathway. Additional key words: differentially expressed genes, GC-MS, HPLC, microarray, Nicotiana http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biologia Plantarum Springer Journals

Dissecting the transcriptional networks underlying the gibberellin response in Nicotiana tabacum

Loading next page...
 
/lp/springer_journal/dissecting-the-transcriptional-networks-underlying-the-gibberellin-thWJLRbCJc
Publisher
Springer Netherlands
Copyright
Copyright © 2018 by The Institute of Experimental Botany
Subject
Life Sciences; Plant Sciences
ISSN
0006-3134
eISSN
1573-8264
D.O.I.
10.1007/s10535-018-0809-0
Publisher site
See Article on Publisher Site

Abstract

The present work examines global transcriptional and limited metabolic profiling to dissect the signalling pathways associated with gibberellin A (GA ) induced alterations in tobacco (Nicotiana tabacum L.) under field conditions. Our 3 3 microarray analysis revealed that GA foliar application elicited 7 032 differentially expressed genes (DEGs) with 3 507 and 3 525 genes displaying up- and down-regulation, respectively. These DEGs broadly belong to diverse metabolic pathways: GA signalling and homeostasis, ethylene and auxin biosyntheses, saccharide metabolism, glycolysis- tricarboxylic acid cycle, terpenoid biosynthesis, shikimate-phenylpropanoid pathway, S-adenosyl methionine cycle, fatty acid biosynthesis, cell wall metabolism, oxidative stress, DNA repair, and stress responses. Transcription validation of representative DEGs was confirmed by real-time quantitative PCR. A relative enhancement in cellular acetyl-CoA pool corroborated with a concomitant increase and decrease in transcriptions as well as activities of histone acetyltransferases and histone deacetylases, respectively. Phytochemical analysis shows an enhanced content of total sugars, reducing sugars, total flavonoids, and phenolics with a concomitant decrease of the content of starch, chlorophylls a and b, and carotenoids. Gas chromatography with mass spectrometry analysis revealed the presence of 30 differential metabolites, mostly belonging to terpenoid biosynthesis pathway. Additional key words: differentially expressed genes, GC-MS, HPLC, microarray, Nicotiana

Journal

Biologia PlantarumSpringer Journals

Published: May 30, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off