Access the full text.
Sign up today, get DeepDyve free for 14 days.
(1999)
A second gene encoding the plastidic pyruvate dehydrogenase β-subunit in Arabidopsis thaliana
Isabelle Lutziger, D. Oliver (2000)
Molecular evidence of a unique lipoamide dehydrogenase in plastids: analysis of plastidic lipoamide dehydrogenase from Arabidopsis thaliana 1FEBS Letters, 484
B. Mooney, J. Miernyk, D. Randall (1999)
Cloning and characterization of the dihydrolipoamide S-acetyltransferase subunit of the plastid pyruvate dehydrogenase complex (E2) from Arabidopsis.Plant physiology, 120 2
F. Kang (1995)
Carbon sources for starch and fatty acid synthesis in plastids from developing embryos of oilseed rape.
Sinéad Drea, R. Mould, J. Hibberd, J. Gray, T. Kavanagh (2001)
Tissue-specific and developmental-specific expression of an Arabidopsis thaliana gene encoding the lipoamide dehydrogenase component of the plastid pyruvate dehydrogenase complexPlant Molecular Biology, 46
V. Hecht, Jean-Philippe Vielle-Calzada, M. Hartog, E. Schmidt, K. Boutilier, U. Grossniklaus, S. Vries (2001)
The Arabidopsis Somatic Embryogenesis Receptor Kinase 1 Gene Is Expressed in Developing Ovules and Embryos and Enhances Embryogenic Competence in CulturePlant Physiology, 127
J. Shellhammer, D. Meinke (1990)
Arrested Embryos from the bio1 Auxotroph of Arabidopsis thaliana Contain Reduced Levels of Biotin.Plant physiology, 93 3
M. Johnston, M. Luethy, J. Miernyk, D. Randall (1997)
Cloning and molecular analyses of the Arabidopsis thaliana plastid pyruvate dehydrogenase subunits.Biochimica et biophysica acta, 1321 3
D. Meinke (1985)
Embryo-lethal mutants of Arabidopsis thaliana: analysis of mutants with a wide range of lethal phasesTheoretical and Applied Genetics, 69
A. Höglund, J. Rödin, Erik Larsson, Lars Rask (1992)
Distribution of napin and cruciferin in developing rape seed embryos.Plant physiology, 98 2
D. Meinke (1995)
Molecular Genetics of Plant Embryogenesis, 46
F. Kang, S. Rawsthorne (1996)
Metabolism of glucose-6-phosphate and utilization of multiple metabolites for fatty acid synthesis by plastids from developing oilseed rape embryosPlanta, 199
S. Clough, A. Bent (1998)
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.The Plant journal : for cell and molecular biology, 16 6
Tama Schneider, Randy Dinkins, Ken Robinson, J. Shellhammer, D. Meinke (1989)
An embryo-lethal mutant of Arabidopsis thaliana is a biotin auxotroph.Developmental biology, 131 1
Bao, Pollard, Ohlrogge (1998)
The biosynthesis of erucic acid in developing embryos of brassica rapaPlant physiology, 118 1
R. Behal, Ming Lin, Stephanie Back, D. Oliver (2002)
Role of acetyl-coenzyme A synthetase in leaves of Arabidopsis thaliana.Archives of biochemistry and biophysics, 402 2
Jingdong Sun, Jinshan Ke, Jerry, 1., Johnson, Basil, Nikolau, E. Wurtele (1997)
Biochemical and Molecular Biological Characterization of CAC2, the Arabidopsis thaliana Gene Coding for the Biotin Carboxylase Subunit of the Plastidic Acetyl-Coenzyme A Carboxylase, 115
Jinshan Ke, R. Behal, Stephanie Back, B. Nikolau, E. Wurtele, David Oliver (2000)
The role of pyruvate dehydrogenase and acetyl-coenzyme A synthetase in fatty acid synthesis in developing Arabidopsis seeds.Plant physiology, 123 2
F. Kang, S. Rawsthorne (1994)
Starch and fatty acid synthesis in plastids from developing embryos of oilseed rape (Brassica napus L.)Plant Journal, 6
F. Kang, S. Rawsthorne (1994)
Starch and fatty acid synthesis in plastids from developing embryos of oilseed rapePlant J., 6
B. Mooney, J. Miernyk, D. Randall (2002)
The complex fate of alpha-ketoacids.Annual review of plant biology, 53
J. Thelen, M. Muszynski, J. Miernyk, D. Randall (1998)
Molecular Analysis of Two Pyruvate Dehydrogenase Kinases from Maize*The Journal of Biological Chemistry, 273
Jinshan Ke, Joong-Kook Choi, Marianne Smith, H. Horner, B. Nikolau, E. Wurtele (1997)
Structure of the CAC1 Gene and in Situ Characterization of Its Expression (The Arabidopsis thaliana Gene Coding for the Biotin-Containing Subunit of the Plastidic Acetyl-Coenzyme A Carboxylase), 113
G. Sunilkumar, LeAnne Mohr, Emily Lopata-Finch, Chandrakanth Emani, K. Rathore (2002)
Developmental and tissue-specific expression of CaMV 35S promoter in cotton as revealed by GFPPlant Molecular Biology, 50
B.P. Mooney, J.A. Miernyk, D.D. Randall (2002)
The complex fate of β-ketoacidsAnnu. Rev. Plant Biol., 53
M. Johnston, J. Miernyk, D. Randall (2000)
Import, processing, and assembly of the α- and β-subunits of chloroplast pyruvate dehydrogenasePlanta, 211
D. Patton, Amy Schetter, L. Franzmann, Karin Nelson, E. Ward, D. Meinke (1998)
An embryo-defective mutant of arabidopsis disrupted in the final step of biotin synthesisPlant physiology, 116 3
The pyruvate dehydrogenase multi-enzyme complex is the main source of acetyl-CoA formation in the plastids of plants and is composed of multiple copies of four different subunits, E1α, E1β, E2, and E3. A T-DNA insertion into the gene for the plastidic E2 (dihydrolipoyl acetyltransferase) subunit, plE2, of the complex in Arabidopsis destroys the expression of that gene. The resulting mutation has no apparent phenotype in the heterozygous state, but the homozygous mutation is lethal. Haploid sperm and eggs that contain only the disrupted plE2 gene function normally resulting in the formation of an embryo that is homozygous for the mutation. This embryo only develops to an early stage before the development arrests resulting in an early embryo-lethal phenotype. While the mutation could not be complemented with the cDNA for the plE2 gene under control of the 35S, the AtSERK1, or the napin promoter, it could be complemented using the endogenous plE2 promoter to drive expression of the plE2 cDNA. This verifies the essential nature of the plastidic pyruvate dehydrogenase complex and its role in embryo formation.
Plant Molecular Biology – Springer Journals
Published: Oct 7, 2004
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.