Discovery of stress responsive TATA-box binding protein associated Factor6 (TAF6) from finger millet (Eleusine coracana (L.) Gaertn)

Discovery of stress responsive TATA-box binding protein associated Factor6 (TAF6) from finger... Drought is one of the major abiotic factors affecting plant growth mainly in the tropical regions. During stress acclimation response, basal transcriptional process is critical which is coordinated by several cofactors, among which TATAbox Binding Protein (TBP) - Associated Factors (TAFs) are the major proteins involved in pre-initiation complex assembly. In search of novel transcriptional regulators, we identified one of the factors, TAF6, in a drought cDNA library generated from an adapted crop, finger millet (Eleusine coracana (L.) Gaertn). RTPCR analysis in leaf tissues of finger millet experiencing different levels of drought, revealed its stress responsiveness in three different genotypes. The gene is also induced under salt, osmotic and oxidative stresses at seedling stage. Analysis of its promoter in model plants indicated the presence of cis-elements associated with diverse stress responses. Down regulation of NbTAF6 by virus induced gene silencing (VIGS) approach resulted in a developmentally defective and stress sensitive phenotype in Nicotiana benthamiana. This is the first report on the up-regulation of EcTAF6 under abiotic stresses and the gene probably has a crucial role in safeguarding the stress related transcription process. The basal regulators like TAF6 can thus be considered as prospective candidates associated with stress acclimation in plants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Plant Biology Springer Journals

Discovery of stress responsive TATA-box binding protein associated Factor6 (TAF6) from finger millet (Eleusine coracana (L.) Gaertn)

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Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2017 by Korean Society of Plant Biologists and Springer-Verlag GmbH Germany
Subject
Life Sciences; Plant Sciences; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant Systematics/Taxonomy/Biogeography; Plant Ecology
ISSN
1226-9239
eISSN
1867-0725
D.O.I.
10.1007/s12374-016-0574-6
Publisher site
See Article on Publisher Site

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