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Discovery of proteins associated with a predefined genomic locus via dCas9–APEX-mediated proximity labeling

Discovery of proteins associated with a predefined genomic locus via dCas9–APEX-mediated... Regulation of gene expression is primarily controlled by changes in the proteins that occupy genes’ regulatory elements. We developed genomic locus proteomics (GLoPro), in which we combine CRISPR-based genome targeting, proximity labeling, and quantitative proteomics to discover proteins associated with a specific genomic locus in native cellular contexts. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Methods Springer Journals

Discovery of proteins associated with a predefined genomic locus via dCas9–APEX-mediated proximity labeling

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Publisher
Springer Journals
Copyright
Copyright © 2018 by The Author(s)
Subject
Life Sciences; Life Sciences, general; Biological Techniques; Biological Microscopy; Biomedical Engineering/Biotechnology; Bioinformatics; Proteomics
ISSN
1548-7091
eISSN
1548-7105
DOI
10.1038/s41592-018-0007-1
Publisher site
See Article on Publisher Site

Abstract

Regulation of gene expression is primarily controlled by changes in the proteins that occupy genes’ regulatory elements. We developed genomic locus proteomics (GLoPro), in which we combine CRISPR-based genome targeting, proximity labeling, and quantitative proteomics to discover proteins associated with a specific genomic locus in native cellular contexts.

Journal

Nature MethodsSpringer Journals

Published: May 7, 2018

References