Gene trapping is one of the most promising technologies of vector mutagenesis used for detection of genes active in embryogenesis. A brief description is provided for gene trapping based on the use of embryonic stem cells and vectors carrying the β-gal reporter gene without promoter, as well as the results obtained with the help of this technology. A total of four transgenic mouse lines were obtained, in three of which the vector was integrated into genes active during embryogenesis. As a result, various patterns of β-galactosidase expression were observed in the limb buds, heart, liver, and other organs at different embryonic stages. At present, detailed studies of the site and time of action of the mutated genes in embryonic and postnatal development are under way, as well as molecular-genetic identification of these genes.
Russian Journal of Developmental Biology – Springer Journals
Published: Sep 19, 2004
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