Direct Coupling of Action Potential Generation in Cells of a Higher Plant (Cucurbita pepo) with the Operation of an Electrogenic Pump

Direct Coupling of Action Potential Generation in Cells of a Higher Plant (Cucurbita pepo) with... Changes in membrane potential (E m) were investigated during action potential (AP) generation by excitable cells in bundle parenchyma of 2-week-old pumpkin (Cucurbita pepo L.) seedlings. APs were evoked by gradual cooling from 21 to 6°C at a rate of 1.3–1.5°C/min. Changes in E m were measured with standard microelectrode technique. The plots of the function dE m/dt = f (E m) were obtained from experimental data by numerical differentiation of E m records. The depolarization proceeded as a one-stage process. Conversely, AP repolarization comprised two stages, distinguished by specific dynamics of dE m /dt. The second stage of repolarization occurred on a larger scale of E m and was more sensitive to temperature than the first one. It is supposed that the second stage of repolarization during AP is related to the operation of electrogenic H+-pump in the excitable membrane. The scheme of AP generation in higher plant cells is suggested; it foresees the involvement in AP generation of both passive and active mechanisms of electrogenesis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Direct Coupling of Action Potential Generation in Cells of a Higher Plant (Cucurbita pepo) with the Operation of an Electrogenic Pump

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Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2002 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1023/A:1013732816263
Publisher site
See Article on Publisher Site

Abstract

Changes in membrane potential (E m) were investigated during action potential (AP) generation by excitable cells in bundle parenchyma of 2-week-old pumpkin (Cucurbita pepo L.) seedlings. APs were evoked by gradual cooling from 21 to 6°C at a rate of 1.3–1.5°C/min. Changes in E m were measured with standard microelectrode technique. The plots of the function dE m/dt = f (E m) were obtained from experimental data by numerical differentiation of E m records. The depolarization proceeded as a one-stage process. Conversely, AP repolarization comprised two stages, distinguished by specific dynamics of dE m /dt. The second stage of repolarization occurred on a larger scale of E m and was more sensitive to temperature than the first one. It is supposed that the second stage of repolarization during AP is related to the operation of electrogenic H+-pump in the excitable membrane. The scheme of AP generation in higher plant cells is suggested; it foresees the involvement in AP generation of both passive and active mechanisms of electrogenesis.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 13, 2004

References

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