Changes in membrane potential (E m) were investigated during action potential (AP) generation by excitable cells in bundle parenchyma of 2-week-old pumpkin (Cucurbita pepo L.) seedlings. APs were evoked by gradual cooling from 21 to 6°C at a rate of 1.3–1.5°C/min. Changes in E m were measured with standard microelectrode technique. The plots of the function dE m/dt = f (E m) were obtained from experimental data by numerical differentiation of E m records. The depolarization proceeded as a one-stage process. Conversely, AP repolarization comprised two stages, distinguished by specific dynamics of dE m /dt. The second stage of repolarization occurred on a larger scale of E m and was more sensitive to temperature than the first one. It is supposed that the second stage of repolarization during AP is related to the operation of electrogenic H+-pump in the excitable membrane. The scheme of AP generation in higher plant cells is suggested; it foresees the involvement in AP generation of both passive and active mechanisms of electrogenesis.
Russian Journal of Plant Physiology – Springer Journals
Published: Oct 13, 2004
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