Differential Phosphoinositide Binding to Components of the G Protein-Gated K+ Channel

Differential Phosphoinositide Binding to Components of the G Protein-Gated K+ Channel The regulation of ion channels and transporters by anionic phospholipids is currently very topical. G protein-gated K+ channels from the Kir3.0 family are involved in slowing the heart rate, generating late inhibitory postsynaptic potentials and controlling hormone release from neuroendocrine cells. There is considerable functional precedent for the control of these channels by phosphatidylinositol 4,5-bisphosphate. In this study, we used a biochemical assay to investigate the lipid binding properties of Kir3.0 channel domains. We reveal a differential binding affinity to a range of phosphoinositides between the C termini of the Kir3.0 isoforms. Furthermore, the N terminus in addition to the C terminus of Kir3.4 is necessary to observe binding and is decreased by the mutations R72A, K195A and R196A but not K194A. Protein kinase C phosphorylation of the Kir3.1 C-terminal fusion protein decreases anionic phospholipid binding. The differential binding affinity has functional consequences as the inhibition of homomeric Kir3.1, occurring after M3 receptor activation, recovers over minutes while homomeric Kir3.2 does not. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Differential Phosphoinositide Binding to Components of the G Protein-Gated K+ Channel

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Copyright © 2006 by Springer Science+Business Media, Inc.
Life Sciences; Human Physiology; Biochemistry, general
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