Differential gene expression in Escherichia coli during aerosolization from liquid suspension

Differential gene expression in Escherichia coli during aerosolization from liquid suspension Comparative transcriptome analysis was used to determine the differentially expressed genes in Escherichia coli during aerosolization from liquid suspension. Isogenic mutant studies were then used to examine the potential part played by some of these genes in bacterial survival in the air. Bioaerosols were sampled after 3 min of nebulization, which aerosolized the bacteria from the liquid suspension to an aerosol chamber (A0), and after further 30 min of airborne suspension in the chamber (A30). Bacteria at A0 showed 65 differentially expressed genes (30 downregulated and 35 upregulated) as compared to the original bacteria in the nebulizer. Droplet evaporation models predicted a drop in temperature in the bioaerosols, which coincides with the change in the expression of cold shock protein genes—cspB and cspG in the bacteria. The most notable group of differentially expressed genes was sorbitol transport and metabolism genes (srlABDEMR). Other genes associated with osmotic stress, nutrient limitation, DNA damage, and other stresses were differentially expressed in the bacteria at A0. After further airborne suspension, one gene (ypfM, which encodes a hypothetical protein with unknown function) was downregulated in the bacteria at A30 as compared to those at A0. Finally, isogenic mutants with either the dps or srlA gene deleted (both genes were upregulated at A0) had lower survival than the parental strain, which is a sign of their potential ability to protect the bacteria in the air. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Microbiology and Biotechnology Springer Journals

Differential gene expression in Escherichia coli during aerosolization from liquid suspension

11 pages
Read Article

Loading next page...
 
/lp/springer_journal/differential-gene-expression-in-escherichia-coli-during-aerosolization-sG5y68AOW5
Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer-Verlag GmbH Germany, part of Springer Nature
Subject
Life Sciences; Microbiology; Microbial Genetics and Genomics; Biotechnology
ISSN
0175-7598
eISSN
1432-0614
DOI
10.1007/s00253-018-9083-5
Publisher site
See Article on Publisher Site

Abstract

Comparative transcriptome analysis was used to determine the differentially expressed genes in Escherichia coli during aerosolization from liquid suspension. Isogenic mutant studies were then used to examine the potential part played by some of these genes in bacterial survival in the air. Bioaerosols were sampled after 3 min of nebulization, which aerosolized the bacteria from the liquid suspension to an aerosol chamber (A0), and after further 30 min of airborne suspension in the chamber (A30). Bacteria at A0 showed 65 differentially expressed genes (30 downregulated and 35 upregulated) as compared to the original bacteria in the nebulizer. Droplet evaporation models predicted a drop in temperature in the bioaerosols, which coincides with the change in the expression of cold shock protein genes—cspB and cspG in the bacteria. The most notable group of differentially expressed genes was sorbitol transport and metabolism genes (srlABDEMR). Other genes associated with osmotic stress, nutrient limitation, DNA damage, and other stresses were differentially expressed in the bacteria at A0. After further airborne suspension, one gene (ypfM, which encodes a hypothetical protein with unknown function) was downregulated in the bacteria at A30 as compared to those at A0. Finally, isogenic mutants with either the dps or srlA gene deleted (both genes were upregulated at A0) had lower survival than the parental strain, which is a sign of their potential ability to protect the bacteria in the air.

Journal

Applied Microbiology and Biotechnology – Springer Journals

Published: May 28, 2018

References

  • Hierarchy of non-glucose sugars in Escherichia coli
    Aidelberg, G; Towbin, BD; Rothschild, D; Dekel, E; Bren, A; Alon, U
  • Statistical design and analysis of RNA sequencing data
    Auer, PL; Doerge, RW
  • The entericidin locus of Escherichia coli and its implications for programmed bacterial cell death
    Bishop, RE; Leskiw, BK; Hodges, RS; Kay, CM; Weiner, JH
  • A microarray-based antibiotic screen identifies a regulatory roles for supercoiling in the osmotic stress response of Escherichia coli
    Cheung, KL; Badarinarayana, V; Selinger, DW; Janse, D; Church, GM
  • The toxic effect of oxygen upon the aerosol survival of Escherichia coli B
    Cox, CS; Baldwin, F
  • The lethal effect of relative humidity on air-borne bacteria
    Dunklin, EW; Puck, TT
  • Osmoadaptation mechanisms in prokaryotes: distribution of compatible solutes
    Empadinhas, N; Costa, MS
  • Differential thermoregulation of two highly homologous cold-shock genes, cspA and cspB, of Escherichia coli
    Etchegaray, JP; Jones, PG; Inouye, M
  • Application of the fluorogenic probe technique (Taqman PCR) to the detection of Enterococcus spp. and Escherichia coli in water samples
    Fram, E; Obst, U
  • The role of particle size in aerosolized pathogen transmission: a review
    Gralton, J; Tovey, E; McLaws, ML; Rawlinson, WD
  • Effects of oxygen on aerosolized Serratia marcescens
    Hess, GE
  • A physiological role for DNA supercoiling in the osmotic regulation of gene expression in S. typhimurium and E. coli
    Higgins, CF; Forman, CJ; Stirling, DA; Waddell, L; Booth, IR; May, G; Bremer, E
  • KEGG for linking genomes to life and the environment
    Kanehisa, M; Araki, M; Goto, S; Hattori, M; Hirakawa, M; Itoh, M; Katayama, T; Kawashima, S; Okuda, S; Tokimatsu, T; Yamanishi, Y
  • Over 1000 genes are involved in the DNA damage response of Escherichia coli
    Khil, PP; Camerini-Otero, RD
  • HISAT: a fast spliced aligner with low memory requirements
    Kim, D; Langmead, B; Salzberg, SL
  • Routes for fructose utilization by Escherichia coli
    Kornberg, HL
  • Substrate dependent rRNA production in an airborne bacterium
    Krumins, V; Mainelis, G; Kerkhof, LJ; Fennell, DE
  • Size and UV germicidal irradiation susceptibility of Serratia marcescens when aerosolized from different suspending media
    Lai, KM; Burge, HA; First, MW
  • Ultrafast and memory-efficient alignment of short DNA sequences to the human genome
    Langmead, B; Trapnell, C; Pop, M; Salzberg, SL
  • RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome
    Li, B; Dewey, CN
  • Simulation of airborne microbial droplet transport
    Lighthart, B; Kim, J
  • Evaporation and dispersion of respiratory droplets from coughing
    Liu, L; Wei, J; Li, Y; Ooi, A
  • Type 1 fimbriae, a colonization factor of uropathogenic Escherichia coli, are controlled by the metabolic sensor CRP-cAMP
    Müller, CM; Åberg, A; Straseviçiene, J; Emődy, L; Uhlin, BE; Balsalobre, C
  • Importance of stress-response genes to the survival of airborne Escherichia coli under different levels of relative humidity
    Ng, TW; Chan, WL; Lai, KM
  • Theoretical analysis of the motion and evaporation of exhaled respiratory droplets of mixed composition
    Parienta, D; Morawska, L; Johnson, GR; Ristovski, ZD; Hargreaves, M; Mengersen, K; Corbett, S; Chao, CYH; Katoshevski, D
  • Fast, multiphase volume adaptation to hyperosmotic shock by Escherichia coli
    Pilizota, T; Shaevitz, JW
  • Osmolarity and pH growth conditions regulate fim gene transcription and type 1 pilus expression in uropathogenic Escherichia coli
    Schwan, WR; Lee, JL; Lenard, FA; Matthews, BT; Beck, MT
  • Differential expression in RNA-seq: a matter of depth
    Tarazona, S; Garcia-Alcalde, F; Dopazo, J; Ferrer, A; Conesa, A
  • The cell membrane as a major site of damage during aerosolization of Escherichia coli
    Thomas, RJ; Webber, D; Hopkins, R; Frost, A; Laws, T; Jayasekera, PN; Atkins, T
  • Gene expression profiling of the pH response in Escherichia coli
    Tucker, DL; Tucker, N; Conway, T
  • Cyclic AMP receptor protein (CRP) regulates the expression of cspA, cspB, cspG and cspI, members of cspA family, in Escherichia coli
    Uppal, S; Jawali, N
  • Profiling early osmostress-dependent gene expression in Escherichia coli using DNA macroarrays
    Weber, A; Jung, K
  • Ecological significance of compatible solute accumulation by micro-organisms: from single cells to global climate
    Welsh, DT
  • Low temperature (23°C) increases expression of biofilm-, cold-shock-, and RpoS-dependent genes in Escherichia coli K-12
    White-Ziegler, CA; Um, S; Perez, NM; Berns, AL; Malhowski, AJ; Young, S
  • The CspA family in Escherichia coli: multiple gene duplication for stress adaptation
    Yamanaka, K; Fang, L; Inouye, M
  • WEGO: a web tool for plotting GO annotations
    Ye, J; Fang, L; Zheng, H; Zhang, Y; Chen, J; Zhang, Z; Wang, L; Li, S; Li, R; Bolund, L; Wang, J
  • osmY, a new hyperosmotically inducible gene, encodes a periplasmic protein in Escherichia coli
    Yim, HH; Villarejo, M
  • Membrane lipid homeostasis in bacteria
    Zhang, YM; Rock, CO
  • Release of free DNA by membrane-impaired bacterial aerosols due to aerosolization and air sampling
    Zhen, H; Han, T; Fennel, DE; Mainelis, G

You’re reading a free preview. Subscribe to read the entire article.

Try 2 weeks free now

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

or browse the journals available

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off

Sign up
for free
Start 14 day
Free Trial