Differential expression analysis by cDNA-AFLP of Solanum torvum upon Verticillium dahliae infection

Differential expression analysis by cDNA-AFLP of Solanum torvum upon Verticillium dahliae infection Differentially expressed genes in eggplant (Solanum torvum SW) during root infection with Verticillium dahliae were identified using cDNA-amplified restriction fragment polymorphism (cDNA-AFLP) approach. A total of 140 differential transcript-derived fragments (TDFs) were isolated, in which 79 TDFs were up-regulated, and 34 TDFs were down-regulated after inoculation. BLAST analysis showed that 51% of these TDFs belonged to function-known genes. Further analysis on six TDFs (TDF11, TDF14, TDF16, TDF25, TDF26, and TDF39), which represented different expression patterns, was done. TDF11, encoding cytochrome c oxidase, appeared in 24 h after inoculation; TDF14, encoding retrotransposon protein, disappeared in 6 h after treatment; TDF16, encoding bZIP transcription factor, began to express only in 72 h after inoculation; TDF25, encoding senescence-associated protein, was present between 6 and 24 h; TDF26, encoding cytochrome P450 monooxygenase, and TDF39, encoding metallophosphoesterase, were up-regulated during the entire period after inoculation. Results obtained demonstrated that the defense of S. torvum against V. dahliae is a complex multigene process, which studying is helpful for better understanding verticillium wilt resistance of S. torvum. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Differential expression analysis by cDNA-AFLP of Solanum torvum upon Verticillium dahliae infection

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2010 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Sciences ; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443710050110
Publisher site
See Article on Publisher Site

Abstract

Differentially expressed genes in eggplant (Solanum torvum SW) during root infection with Verticillium dahliae were identified using cDNA-amplified restriction fragment polymorphism (cDNA-AFLP) approach. A total of 140 differential transcript-derived fragments (TDFs) were isolated, in which 79 TDFs were up-regulated, and 34 TDFs were down-regulated after inoculation. BLAST analysis showed that 51% of these TDFs belonged to function-known genes. Further analysis on six TDFs (TDF11, TDF14, TDF16, TDF25, TDF26, and TDF39), which represented different expression patterns, was done. TDF11, encoding cytochrome c oxidase, appeared in 24 h after inoculation; TDF14, encoding retrotransposon protein, disappeared in 6 h after treatment; TDF16, encoding bZIP transcription factor, began to express only in 72 h after inoculation; TDF25, encoding senescence-associated protein, was present between 6 and 24 h; TDF26, encoding cytochrome P450 monooxygenase, and TDF39, encoding metallophosphoesterase, were up-regulated during the entire period after inoculation. Results obtained demonstrated that the defense of S. torvum against V. dahliae is a complex multigene process, which studying is helpful for better understanding verticillium wilt resistance of S. torvum.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Sep 2, 2010

References

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