Differential Effects of Aldosterone and Vasopressin on Chloride Fluxes in Transimmortalized Mouse Cortical Collecting Duct Cells

Differential Effects of Aldosterone and Vasopressin on Chloride Fluxes in Transimmortalized Mouse... The effects of aldosterone and vasopressin on Cl− transport were investigated in a mouse cortical collecting duct (mpkCCD) cell line derived from a transgenic mouse carrying the SV40 large T antigen driven by the proximal regulatory sequences of the L-pyruvate kinase gene. The cells had features of a tight epithelium and expressed the amiloride-sensitive sodium channel and the cystic fibrosis transmembrane conductance regulator (CFTR) genes. dD-arginine vasopressin (dDAVP) caused a rapid, dose-dependent, increase in short-circuit current (I sc ). Experiments with ion channel blockers and apical ion substitution showed that the current represented amiloride-sensitive Na+ and 5-nitro-2-(3-phenylpropylamino)benzoate-sensitive and glibenclamide-sensitive Cl− fluxes. Aldosterone (5 × 10−7 m for 3 or 24 hr) stimulated I sc and apical-to-basal 22Na+ flux by 3-fold. 36Cl− flux studies showed that dDAVP and aldosterone stimulated net Cl− reabsorption and that dDAVP potentiated the action of aldosterone on Cl− transport. Whereas aldosterone affected only the apical-to-basal 36Cl− flux, dDAVP mainly increased the apical-to-basal Cl− flux and the basal-to-apical flux of Cl− to a lesser extent. These results suggest that the discrete dDAVP-elicited Cl− secretion involves the CFTR and that dDAVP and aldosterone may affect in different ways the observed increased Cl− reabsorption in this model of mouse cultured cortical collecting duct cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Differential Effects of Aldosterone and Vasopressin on Chloride Fluxes in Transimmortalized Mouse Cortical Collecting Duct Cells

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Copyright © Inc. by 1998 Springer-Verlag New York
Life Sciences; Biochemistry, general; Human Physiology
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