The Arabidopsis phyB, phyD, and phyE phytochromes regulate plant developmental and growth responses to continuous red light and to the ratio of red to far-red light. They are also more highly related in sequence to each other and more recently derived evolutionarily than phyA and phyC. In order to directly compare the signaling activities of these three photoreceptor apoproteins, an assay was developed based upon complementation of the phyB-1 null mutant phenotype with transgenes consisting of the PHYB promoter (PB) driving expression of the PHYB, PHYD, or PHYE coding sequences. Expression analysis indicates that the PB-phyB, PB-phyD, and PB-phyE transgenes are transcriptionally and translationally active. However, whereas the PB-phyB transgene complements the phyB-1 red light hypocotyl growth phenotype completely, the PB-phyD and PB-phyE transgenes are only weakly active in restoring seedling growth regulation. Red light fluence curves indicate that this difference is not likely to be due to differences in dark reversion rates. The PB-phyD and PB-phyE transgenes also both partially restore the rosette leaf morphology phenotype of the phyB-1 mutant. However, the PB-phyD gene complements the early flowering phenotype of phyB-1 very strongly whereas PB-phyE does not. These results demonstrate that Arabidopsis phyB-related apoproteins differ significantly in their capacities to signal in various seedling and adult plant phytochrome responses.
Plant Molecular Biology – Springer Journals
Published: Oct 7, 2004
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera