Different expression patterns of CK2 subunits in the brains of experimental animals and patients with transmissible spongiform encephalopathies

Different expression patterns of CK2 subunits in the brains of experimental animals and patients... To address the possible alteration of casein kinase 2 (CK2) in transmissible spongiform encephalopathies (TSEs), the levels and patterns of CK2 in the brain tissues of hamsters or C57BL mice inoculated intracerebrally with scrapie agents 263K or 139A were evaluated by Western blots, followed by quantitative analysis. Specific semi-quantitative RT-PCR for evaluating the mRNA transcripts of CK2 subunits was performed in parallel. Compared with normal animals, the levels of CK2α and CK2β in the brains of infected hamsters and mice were significantly decreased, regardless of which scrapie agent was. However, the expression of CK2α′ or CK2α′/CK2α′′ in the animals infected with agents 263K or 139A was considerably increased. Furthermore, decreases of CK2α and CK2β and increases of CK2α′/CK2α′′ were observed in cerebella homogenates from one familial Creutzfeldt-Jakob disease (fCJD) case and one fatal familial insomnia (FFI) case. These results suggest that alterations of CK2 subunits in brains are illness-correlative phenomena in TSEs and indicate a potential linkage of CK2 changes with the pathogenesis of prion diseases. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Different expression patterns of CK2 subunits in the brains of experimental animals and patients with transmissible spongiform encephalopathies

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Publisher
Springer Vienna
Copyright
Copyright © 2008 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-008-0084-z
Publisher site
See Article on Publisher Site

Abstract

To address the possible alteration of casein kinase 2 (CK2) in transmissible spongiform encephalopathies (TSEs), the levels and patterns of CK2 in the brain tissues of hamsters or C57BL mice inoculated intracerebrally with scrapie agents 263K or 139A were evaluated by Western blots, followed by quantitative analysis. Specific semi-quantitative RT-PCR for evaluating the mRNA transcripts of CK2 subunits was performed in parallel. Compared with normal animals, the levels of CK2α and CK2β in the brains of infected hamsters and mice were significantly decreased, regardless of which scrapie agent was. However, the expression of CK2α′ or CK2α′/CK2α′′ in the animals infected with agents 263K or 139A was considerably increased. Furthermore, decreases of CK2α and CK2β and increases of CK2α′/CK2α′′ were observed in cerebella homogenates from one familial Creutzfeldt-Jakob disease (fCJD) case and one fatal familial insomnia (FFI) case. These results suggest that alterations of CK2 subunits in brains are illness-correlative phenomena in TSEs and indicate a potential linkage of CK2 changes with the pathogenesis of prion diseases.

Journal

Archives of VirologySpringer Journals

Published: Jun 1, 2008

References

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