Differences in transmissibility and pathogenicity of reassortants between H9N2 and 2009 pandemic H1N1 influenza A viruses from humans and swine

Differences in transmissibility and pathogenicity of reassortants between H9N2 and 2009 pandemic... Both H9N2 subtype avian influenza and 2009 pandemic H1N1 viruses (pH1N1) can infect humans and pigs, which provides the opportunity for virus reassortment, leading to the genesis of new strains with potential pandemic risk. In this study, we generated six reassortant H9 viruses in the background of three pH1N1 strains from different hosts (A/California/04/2009 (CA04), A/Swine/Jiangsu/48/2010 (JS48) and A/Swine/Jiangsu/285/2010 (JS285)) by replacing either the HA (H9N1-pH1N1) or both the HA and NA genes (H9N2-pH1N1) from an h9.4.2.5-lineage H9N2 subtype influenza virus, A/Swine/Taizhou/5/08 (TZ5). The reassortant H9 viruses replicated to higher titers in vitro and in vivo and gained both efficient transmissibility in guinea pigs and increased pathogenicity in mice compared with the parental H9N2 virus. In addition, differences in transmissibility and pathogenicity were observed among these reassortant H9 viruses. The H9N2-pH1N1viruses were transmitted more efficiently than the corresponding H9N1-pH1N1 viruses but showed significantly decreased pathogenicity. One of the reassortant H9 viruses that were generated, H9N-JS48, showed the highest virulence in mice and acquired respiratory droplet transmissibility between guinea pigs. These results indicate that coinfection of swine with H9N2 and pH1N1viruses may pose a threat for humans if reassortment occurs, emphasizing the importance of surveillance of these viruses in their natural hosts. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Differences in transmissibility and pathogenicity of reassortants between H9N2 and 2009 pandemic H1N1 influenza A viruses from humans and swine

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Publisher
Springer Vienna
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-014-2009-3
Publisher site
See Article on Publisher Site

Abstract

Both H9N2 subtype avian influenza and 2009 pandemic H1N1 viruses (pH1N1) can infect humans and pigs, which provides the opportunity for virus reassortment, leading to the genesis of new strains with potential pandemic risk. In this study, we generated six reassortant H9 viruses in the background of three pH1N1 strains from different hosts (A/California/04/2009 (CA04), A/Swine/Jiangsu/48/2010 (JS48) and A/Swine/Jiangsu/285/2010 (JS285)) by replacing either the HA (H9N1-pH1N1) or both the HA and NA genes (H9N2-pH1N1) from an h9.4.2.5-lineage H9N2 subtype influenza virus, A/Swine/Taizhou/5/08 (TZ5). The reassortant H9 viruses replicated to higher titers in vitro and in vivo and gained both efficient transmissibility in guinea pigs and increased pathogenicity in mice compared with the parental H9N2 virus. In addition, differences in transmissibility and pathogenicity were observed among these reassortant H9 viruses. The H9N2-pH1N1viruses were transmitted more efficiently than the corresponding H9N1-pH1N1 viruses but showed significantly decreased pathogenicity. One of the reassortant H9 viruses that were generated, H9N-JS48, showed the highest virulence in mice and acquired respiratory droplet transmissibility between guinea pigs. These results indicate that coinfection of swine with H9N2 and pH1N1viruses may pose a threat for humans if reassortment occurs, emphasizing the importance of surveillance of these viruses in their natural hosts.

Journal

Archives of VirologySpringer Journals

Published: Jul 1, 2014

References

  • A review of avian influenza in different bird species
    Alexander, DJ
  • Experimental adaptation of an influenza H5 HA confers respiratory droplet transmission to a reassortant H5 HA/H1N1 virus in ferrets
    Imai, M; Watanabe, T; Hatta, M; Das, SC; Ozawa, M; Shinya, K; Zhong, G; Hanson, A; Katsura, H; Watanabe, S; Li, C; Kawakami, E; Yamada, S; Kiso, M; Suzuki, Y; Maher, EA; Neumann, G; Kawaoka, Y
  • Novel H1N2 swine influenza reassortant strain in pigs derived from the pandemic H1N1/2009 virus
    Moreno, A; Trani, L; Faccini, S; Vaccari, G; Nigrelli, D; Boniotti, MB; Falcone, E; Boni, A; Chiapponi, C; Sozzi, E; Cordioli, P
  • Escape mutants of pandemic influenza A/H1N1 2009 virus: variations in antigenic specificity and receptor affinity of the hemagglutinin
    Rudneva, I; Ignatieva, A; Timofeeva, T; Shilov, A; Kushch, A; Masalova, O; Klimova, R; Bovin, N; Mochalova, L; Kaverin, N
  • Origins and evolutionary genomics of the 2009 swine-origin H1N1 influenza A epidemic
    Smith, GJ; Vijaykrishna, D; Bahl, J; Lycett, SJ; Worobey, M; Pybus, OG; Ma, SK; Cheung, CL; Raghwani, J; Bhatt, S; Peiris, JS; Guan, Y; Rambaut, A
  • Isolation and genetic characterization of avian origin H9N2 influenza viruses from pigs in China
    Yu, H; Hua, RH; Wei, TC; Zhou, YJ; Tian, ZJ; Li, GX; Liu, TQ; Tong, GZ

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