Dexamethasone treatment in vitro resulted in different responses of two fractions of phagocytes of the holothurian Eupentacta fraudatrix

Dexamethasone treatment in vitro resulted in different responses of two fractions of phagocytes... Echinoderm phagocytes are considered to be analogues to vertebrate macrophages. Previously, the phagocytes of some echinoderm species were divided into two fractions with unclearly identified functional properties. This study aims at modeling the immune response of two phagocyte fractions (P1 and P2) of the holothurian Eupentacta fraudatrix to the synthetic glucocorticoid hormone dexamethasone (Dex) in vitro and at comparison of the effects of such pretreatment on humoral cooperation of each phagocyte fraction with another type of immunocytes, morula cells. During 48-h incubation, Dex (0.1–100 μM) induced apoptosis in a direct (in the P1 fraction) or reverse (in the P2 fraction) concentration-dependent manner. In addition, 100 μM Dex differently affected the cytokin-like substance level in the P1 and P2 phagocyte fractions. Moreover, the supernatants of the Dex(100 μM)-pretreated phagocytes induced opposite changes in the IL-1-like substance level in morula cells. These results indicate a striking functional difference between the two phagocyte fractions. The data obtained provide a new insight into the evolution of macrophage response and into the prospects of the use of in vitro holothurian phagocyte model. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Marine Biology Springer Journals

Dexamethasone treatment in vitro resulted in different responses of two fractions of phagocytes of the holothurian Eupentacta fraudatrix

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Publisher
Springer Journals
Copyright
Copyright © 2015 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Freshwater & Marine Ecology
ISSN
1063-0740
eISSN
1608-3377
D.O.I.
10.1134/S1063074015060024
Publisher site
See Article on Publisher Site

Abstract

Echinoderm phagocytes are considered to be analogues to vertebrate macrophages. Previously, the phagocytes of some echinoderm species were divided into two fractions with unclearly identified functional properties. This study aims at modeling the immune response of two phagocyte fractions (P1 and P2) of the holothurian Eupentacta fraudatrix to the synthetic glucocorticoid hormone dexamethasone (Dex) in vitro and at comparison of the effects of such pretreatment on humoral cooperation of each phagocyte fraction with another type of immunocytes, morula cells. During 48-h incubation, Dex (0.1–100 μM) induced apoptosis in a direct (in the P1 fraction) or reverse (in the P2 fraction) concentration-dependent manner. In addition, 100 μM Dex differently affected the cytokin-like substance level in the P1 and P2 phagocyte fractions. Moreover, the supernatants of the Dex(100 μM)-pretreated phagocytes induced opposite changes in the IL-1-like substance level in morula cells. These results indicate a striking functional difference between the two phagocyte fractions. The data obtained provide a new insight into the evolution of macrophage response and into the prospects of the use of in vitro holothurian phagocyte model.

Journal

Russian Journal of Marine BiologySpringer Journals

Published: Jan 5, 2016

References

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