Various aspects of plant development and stress physiology are mediated by Ca2+ signaling. Ca2+ sensors, such as calmodulin, detect these signals and direct downstream signaling pathways by binding and activating diverse targets. Plants possess many unique, putative Ca2+ sensors, including a large family (50 in Arabidopsis) of calmodulin-like proteins termed CMLs. Some of these CMLs have been implicated in Ca2+-based stress response but most remain unstudied. We generated transgenic plants expressing CML::GUS reporter genes for members of a subfamily of CMLs (CML37, CML38 and CML39) which allowed us to investigate their expression patterns in detail. We found that CML::GUS genes displayed unique tissue, cell-type, and temporal patterns of expression throughout normal development, particularly in the flower, and in response to a variety of stimuli, including biotic and abiotic stress, hormone and chemical treatments. Our findings are supported by semiquantitative reverse-transcription PCR as well as analyses of microarray databases. Analysis of purified, recombinant CMLs demonstrated their ability to bind Ca2+ in vitro. Collectively, our data suggest that these CMLs likely play important roles as sensors in Ca2+-mediated developmental and stress response pathways and provide a framework of spatial and temporal expression to direct future studies aimed at elucidating their physiological roles.
Plant Molecular Biology – Springer Journals
Published: Jun 20, 2007
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera