Development of PCR-based CNP marker of rice Waxy gene with confronting two-pair primers

Development of PCR-based CNP marker of rice Waxy gene with confronting two-pair primers Rice amylose content (AC) is a key determinant for grain end-use quality attributes. The base substitution ((G → T) at +1 loci of the first intron (In1) of Waxy (Wx), a major gene controlling AC in rice, results in decreased AC. A new SNP typing method of Wx In1 based on polymerase chain reaction with confronting two-pair primers (PCR-CTPP) was reported here: first its practicability was confirmed by 23 varieties with known SNP and AC; and then the segregation ratio at target SNP loci were checked and it fitted well for 1: 2: 1 single gene segregation; at last SNP typing and AC assay with 150 mini core collections (MCC) in China showed that average AC of 53 G type varieties (22.5%) was significantly higher than that of 97 T type varieties (13.7%) (p < 0.01) and the target SNP loci explained 77.8% AC variation. So this method could be used to estimate AC of rice variety roughly or in marker-assisted breeding, that is, using variety with known and desired AC as Wx allele donor parent and aided with crossbreed, backcross and marker-assisted selection (MAS) reported here, rice breeders could improve AC of varieties with comprehensively excellent performance to meet special end-products. Russian Journal of Genetics Springer Journals

Development of PCR-based CNP marker of rice Waxy gene with confronting two-pair primers

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Pleiades Publishing
Copyright © 2015 by Pleiades Publishing, Inc.
Biomedicine; Human Genetics; Animal Genetics and Genomics; Microbial Genetics and Genomics
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