The changes in chlorophyll and proline contents, the rate of photosynthetic oxygen evolution, the activity of superoxide dismutase (SOD), and NADPH concentration in the cells of Chlorella stigmatophoraButcher were followed under the conditions when the glycolate pathway was inhibited by 10 mM α-hydroxy-2-pyridinemethane sulfonate + 10 mM isonicotinoyl hydrazine or with 425 mM NaCl. The oxidative stress exerted by the inhibition of the glycolate pathway developed in three phases. At the first phase (15 to 60 min), the photosynthetic rate slightly increased, chlorophyll and proline contents and SOD activity declined, whereas NADPH pool increased considerably. At the second phase (1 to 7 h), in addition to a short-period chlorophyll accumulation, proline synthesis enhanced together with SOD activity, whereas NADPH pool decreased. Degradation processes dominated the third phase (24 to 72 h): cells absorbed rather than evolved oxygen, and the contents of proline, chlorophyll, and NADPH as well as the SOD activity decreased dramatically. The authors conclude that the inhibition of photorespiration in chlorella cells disrupted the functions of the electron transport chain (ETC); as a result, the oxygen stress developed, and cells became bleached. The accumulation of free proline in the cells under salinization provides a mechanism subduing ETC overreduction, and photorespiration is a component of the cell antioxidant system.
Russian Journal of Plant Physiology – Springer Journals
Published: Oct 10, 2004
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