Development of new mitochondrial DNA markers in Scots pine (Pinus sylvestris L.) for population and phylogeographic studies

Development of new mitochondrial DNA markers in Scots pine (Pinus sylvestris L.) for population... Fragments of genomic DNA of Scots pine (Pinus sylvestris L.) homologous to the mitochondrial DNA (mtDNA) contigs of Norway spruce (Picea abies (L.) Karst.) and loblolly pine (Pinus taeda L.) were resequenced in a sample of the Scots pine trees of European, Siberian, Mongolian, and Caucasian origin in order to develop mtDNA markers. Flanking non-coding regions of some mitochondrial genes were also investigated and resequenced. Five single nucleotide polymorphisms (SNPs) and a single minisatellite locus were identified. Caucasian samples differed from the rest by three SNPs. Two SNPs have been linked to an early described marker in the first intron of the nad7 gene, and all together revealed three haplotypes in European populations. No variable SNPs were found in the Siberian and Mongolian populations. The minisatellite locus contained 41 alleles across European, Siberian, and Mongolian populations, but, this locus demonstrated a weak population differentiation (F ST = 5.8), probably due to its high mutation rate. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Development of new mitochondrial DNA markers in Scots pine (Pinus sylvestris L.) for population and phylogeographic studies

5 pages
Read Article

Loading next page...
 
/lp/springer_journal/development-of-new-mitochondrial-dna-markers-in-scots-pine-pinus-Yv2xolw6Z9
Publisher
Springer Journals
Copyright
Copyright © 2015 by Pleiades Publishing, Inc.
Subject
Biomedicine; Human Genetics; Animal Genetics and Genomics; Microbial Genetics and Genomics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795415120108
Publisher site
See Article on Publisher Site

Abstract

Fragments of genomic DNA of Scots pine (Pinus sylvestris L.) homologous to the mitochondrial DNA (mtDNA) contigs of Norway spruce (Picea abies (L.) Karst.) and loblolly pine (Pinus taeda L.) were resequenced in a sample of the Scots pine trees of European, Siberian, Mongolian, and Caucasian origin in order to develop mtDNA markers. Flanking non-coding regions of some mitochondrial genes were also investigated and resequenced. Five single nucleotide polymorphisms (SNPs) and a single minisatellite locus were identified. Caucasian samples differed from the rest by three SNPs. Two SNPs have been linked to an early described marker in the first intron of the nad7 gene, and all together revealed three haplotypes in European populations. No variable SNPs were found in the Siberian and Mongolian populations. The minisatellite locus contained 41 alleles across European, Siberian, and Mongolian populations, but, this locus demonstrated a weak population differentiation (F ST = 5.8), probably due to its high mutation rate.

Journal

Russian Journal of Genetics – Springer Journals

Published: Dec 16, 2015

References

  • Tandem repeats in plant mitochondrial genomes: application to the analysis of population differentiation in the conifer Norway spruce
    Sperisen, C.; Büchler, U.; Gugerli, F.
  • Genetic consequences of glacial survival and postglacial colonization in Norway spruce: combined analysis of mitochondrial DNA and fossil pollen
    Tollefsrud, M.M.; Kissling, R.; Gugerli, F.
  • Combined analysis of nuclear and mitochondrial markers provide new insight into the genetic structure of North European Picea abies
    Tollefsrud, M.M.; Sonstebo, J.H.; Brochmann, C.
  • Glacial survival of boreal trees in northern Scandinavia
    Parducci, L.; Jorgensen, T.; Tollefsrud, M.M.
  • Cytoplasmic DNA variation and biogeography of Larix Mill. in Northeast Asia
    Polezhaeva, M.A.; Lascoux, M.; Semerikov, V.L.
  • Southern montane populations did not contribute to the recolonization of West Siberian Plain by Siberian larch (Larix sibirica): a range-wide analysis of cytoplasmic markers
    Semerikov, V.L.; Semerikova, S.A.; Polezhaeva, M.M.
  • Glacial vicariance in Eurasia: mitochondrial DNA evidence from scots pine for complex heritage involving genetically distinct refugia at mid-northern latitudes and in Asia Minor
    Naydenov, K.; Senneville, S.; Beaulieu, J.
  • Colonozation routes of Pinus sylvestris inferred from distribution of mitochondrial DNA variation
    Pyhäjärvi, T.; Salmela, M.; Savolainen, O.
  • Spread of mitochondrial DNA haplotypes in population of scots pine (Pinus sylvestris L.) in northern European Russia
    Vidyakin, A.I.; Semerikov, V.L.; Polezhaeva, M.A.; Dymshakova, O.S.
  • Molecular Cloning: A Laboratory Manual
    Sambrook, J.; Fritsch, E.F.; Maniatis, T.
  • in Bioinformatics Methods and Protocols: Methods in Molecular Biology
    Rozen, S.; Skaletsky, H.J.
  • Preparative and analytical purification of DNA from agarose
    Voglestein, B.; Gillespie, D.
  • BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT
    Hall, T.A.
  • Microsatellite loci polymorphism of chloroplast DNA of scots pine (Pinus sylvestris L.) in Asia and eastern Europe
    Semerikov, V.L.; Semerikova, S.A.; Dymshakova, O.S.
  • Measuring and testing genetic differentiation with ordered versus unordered alleles
    Pons, O.; Petit, R.J.
  • ARLEQUIN ver. 3.1: An Integrated Software Package for Population Genetics Data Analysis. Computational and Molecular Population Genetics Lab (CMPG)
    Excoffier, L.; Laval, G.; Schneider, S.
  • An exact test for population differentiation
    Raymond, M.; Rousset, F.
  • GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism
    Raymond, M.; Rousset, F.
  • in Molecular Systematics of Plants
    Palmer, J.D.
  • Mitochondrial DNA capture and divergence in Pinus provide new insights into the evolution of the genus
    Wang, B.S.; Wang, X.R.

You’re reading a free preview. Subscribe to read the entire article.

Try 2 weeks free now

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

or browse the journals available

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off

Sign up
for free
Start 14 day
Free Trial