The fertilized loach eggs were injected, before the beginning of cleavage, with the nuclear dye Hoechst 33258 and left to develop until the late blastula stage. Some cells of the dorsal area of stained blastoderm were transplanted in the analogous area of intact embryos of the same age, which led to an earlier and more pronounced development of head and trunk structures in recipients. A relationship was established between specific features of the development of recipients and localization of descendants of the transplanted cells. Transplantation of cells of the dorsal area of stained blastoderm in the ventral area of embryos of the same age led to the formation of two axial complexes, both at the same level of development, but behind the control, and stained cells were located predominantly in one of twin embryos.
Russian Journal of Developmental Biology – Springer Journals
Published: Oct 18, 2004
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