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RA Collins, KF Armstrong, AJ Holyoake (2013)
Something in the water: biosecurity monitoring of ornamental fish imports using environmental DNABiol Invasions, 15
F Pleijel, U Jondelius, E Norlinder (2008)
Phylogenies without roots? A plea for the use of vouchers in molecular phylogenetic studiesMol Phylogenet Evol, 48
PL Fuller, LG Nico, JD Williams (1999)
Nonindigenous fishes introduced into inland waters of the United States
PF Thomsen, J Kielgast, LL Iversen (2012)
Detection of a diverse marine fIsh fauna using environmental DNA from seawater samplesPLoS ONE, 7
PF Thomsen, J Kielgast, LL Iversen (2012)
Monitoring endangered freshwater biodiversity using environmental DNAMol Ecol, 21
AM Bolger (2014)
2114Bioinformatics, 30
SF Li, JW Xu, QL Yang (2009)
A comparaison of complete mitochondrial genomes of silver carp Hypophthalmichthys molitrix and bighead carp Hypophthalmichthys nobilis: implicatios for their taxonomic relationship and phylogenyJ Fish Biol, 74
E Diaz-Ferguson, J Herod, J Galvez (2014)
Development of molecular markers for eDNA detection of the invasive African jewelfish (Hemichromis letourneuxi): a new tool for monitoring aquatic invasive species in National Wildlife RefugesManag Biol Invasions, 5
T Dejean, A Valentini, A Duparc (2011)
Persistence of environmental DNA in freshwater ecosystemsPLoS ONE, 6
RP Kelly, JA Port, KM Yamahara (2014)
Using environmental DNA to census marine fishes in a large mesocosmPLoS ONE, 9
CL Jerde, AR Mahon, WL Chadderton (2011)
’’Sight-unseen’’ detection of rare aquatic species using environmental DNAConserv Lett, 4
HL Farrington, CE Edwards, X Guan (2015)
Mitochondrial genome sequencing and development of genetic markers for the detection of DNA of invasive bighead and silver carp (Hypophthalmichthys nobilis and H. molitrix) in environmental water samples from the United StatesPLoS ONE, 10
FJ Rahel (2002)
Homogenization of freshwater faunasAnnu Rev Ecol Evol Syst, 33
TM Wilcox, KS McKelvey, MK Young (2013)
Robust detection of rare species using environmental DNA: the importance of primer specificityPLoS ONE, 8
CS Kolar (2004)
Risk assessment and screening for potentially invasive fishesNew Zealand J Mar Freshw Res, 38
D Steinke, TS Zemlak, PDN Hebert (2009)
Barcoding Nemo: DNA-based identifications for the ornemental fish tradePLoS ONE, 4
RD Ward, TS Zemlak, BH Innes (2005)
DNA barcoding Australia’s fish speciesPhilos Trans R Soc Lond B Biol Sci, 360
DG Elliott, RJ Pascho (2001)
Evidence that coded-wire-tagging procedures can enhance transmission of Renibacterium salmoninarum in Chinook salmonJ Aquat Anim Health, 13
AR Mahon, CL Jerde, M Galaska (2013)
Validation of eDNA surveillance sensitivity for detection of Asian carps in controlled and field experimentsPLoS ONE, 8
X Pochon, NJ Bott, KF Smith (2013)
Evaluating detection limits of next-generation sequencing for the surveillance and monitoring of international marine pestsPLoS ONE, 8
T Minamoto, H Yamanaka, T Takahara (2012)
Surveillance of fish species composition using environmental DNALimnology, 13
CR Turner, DJ Miller, KJ Coyne (2014)
Improved methods for capture, extraction, and quantitative assay of environmental DNA from Asian bigheaded carp (Hypophthalmichthys spp.)PLoS ONE, 9
AM Bolger, M Lohse, B Usadel (2014)
Trimmomatic: a flexible trimmer for Illumina sequence dataBioinformatics, 30
RD Ward, R Hanner, PDN Hebert (2009)
The campaign to DNA barcode all fishes, FISH-BOLJ Fish Biol, 74
LP Hansen (1988)
Effects of Carlin tagging and fin clipping on survival of Atlantic salmon (Salmo salar L.) released as smoltsAquaculture, 70
JS Nelson, TC Grande, MVH Wilson (2016)
Fishes of the world
PDN Hebert, A Cywinska, SL Ball (2003)
Biological identifications through DNA barcodesProc R Soc Lond Ser B Biol Sci, 270
WJ Chen, C Bonillo, G Lecointre (2003)
Repeatability of clades as a criterion of reliability: a case study for molecular phylogeny of Acanthomorpha (Teleostei) with larger number of taxaMol Phylogenet Evol, 26
H Gerson, B Cudmore, NE Mandrak (2008)
Monitoring international wildlife trade with coded species dataConserv Biol, 22
K Han, B Ely (2002)
Use of AFLP analyses to assess genetic variation in Morone and Thunnus speciesMar Biotechnol, 4
RE Gozlan, S St-Hilaire, SW Feist (2005)
Disease threat to European fishNature, 435
C Wilson, E Wright, J Bronnenhuber (2014)
Tracking ghosts: combined electrofishing and environmental DNA surveillance efforts for Asian carps in Ontario waters of Lake ErieManag Biol Invasions, 5
JT Lamer, CR Dolan, JL Petersen (2010)
Introgressive hybridization between bighead carp and silver carp in the Mississippi and Illinois RiversNorth Am J Fish Manag, 30
ES Wright, KH Vetsigian (2016)
Quality filtering of Illumina index reads mitigates sample cross-talkBMC Genomics, 17
N Hubert, R Hanner, E Holm (2008)
Identifying Canadian freshwater fishes through DNA barcodesPLoS ONE, 3
CAM Rixon, IC Duggan, NMN Bergeron (2005)
Invasion risks posed by the aquarium trade and live fish markets on the Laurential Great LakesBiodivers Conserv, 14
RG Sevilla, A Diez, M Norén (2007)
Primers and polymerase chain reaction conditions for DNA barcoding teleost fish based on the mitochondrial cytochrome b and nuclear rhodopsin genesMol Ecol Notes, 7
NE Mandrak, B Cudmore (2010)
The fall of native fishes and the rise of non-native fishes in the Great Lakes BasinAquat Ecosyst Health Manag, 13
RA Collins, KF Armstrong, R Meier (2012)
Barcoding and border biosecurity: identifying cyprinid fishes in the aquarium tradePLoS ONE, 7
M Hajibabaei, S Shokralla, X Zhou (2011)
Environmental barcoding: a next-generation sequencing approach for biomonitoring applications using river benthosPLoS ONE, 6
Preventing the arrival, establishment, and spread of aquatic invasive species is an important step in protecting our aquatic environments. The use of detection tools, like DNA barcoding technologies, high-throughput sequencing and environmental DNA (eDNA) monitoring, is becoming increasingly important in preventing the introduction of potential invasive species. The combination of eDNA with realtime PCR (qPCR) provide the opportunity to have a rapid and specific detection. In this study, we developed a DNA sequence library that has sufficient depth and species coverage such that high-risk species can be confidently discriminated from legitimately imported and native species. A total of 12 species-specific qPCR assays were developed for the detection of 13 potential invasive species (pAIS) in bulk water samples. Detection of these species was also compared with a HTS approach. We have demonstrated the high sensitivity of qPCR assays using eDNA at very low densities, suggesting we could detect a low number of individuals mixed with non-target species in a simulated live shipment. For the detection of a targeted list of species, qPCR is advantageous. The mini-barcodes developed in this project offered a good sensitivity of detection, and HTS is a discovery tool that can be desirable when unlisted or numerous species need to be identified.
Biological Invasions – Springer Journals
Published: Aug 4, 2017
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