Development of boron-efficient near isogenic lines of Brassica napus and their response to low boron stress at seedling stage

Development of boron-efficient near isogenic lines of Brassica napus and their response to low... Rapeseed (Brassica napus) is sensitive to low boron (B) stress and plentiful variation exists in response to B deficiency. One major QTL, BE1, and three minor loci controlling B efficiency in Brassica napus were previously detected. To fine map and clone the B-efficient gene (s), the development of B-efficient NILs in Brassica napus was conducted, combining the identification of B efficiency at seedling stage with genetic background selection using random AFLP markers. The molecular marker assisted background selection proved its optimum and necessary in an early backcrossing generation to select the backcross individuals with high genetic background similarity to accelerate the construction of NILs. Based on B efficiency investigated at seedling stage under the low B conditions, the B-efficient backcross line can produce biomass twice about the B-inefficient parent’s and show low B concentration and effective utilization of B under low B condition. Thus, the B efficiency might be attributed to the higher B utilization efficiency or less demand for B. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Development of boron-efficient near isogenic lines of Brassica napus and their response to low boron stress at seedling stage

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Publisher
Springer Journals
Copyright
Copyright © 2010 by Pleiades Publishing, Ltd.
Subject
Biomedicine; Microbial Genetics and Genomics; Animal Genetics and Genomics; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795410010096
Publisher site
See Article on Publisher Site

Abstract

Rapeseed (Brassica napus) is sensitive to low boron (B) stress and plentiful variation exists in response to B deficiency. One major QTL, BE1, and three minor loci controlling B efficiency in Brassica napus were previously detected. To fine map and clone the B-efficient gene (s), the development of B-efficient NILs in Brassica napus was conducted, combining the identification of B efficiency at seedling stage with genetic background selection using random AFLP markers. The molecular marker assisted background selection proved its optimum and necessary in an early backcrossing generation to select the backcross individuals with high genetic background similarity to accelerate the construction of NILs. Based on B efficiency investigated at seedling stage under the low B conditions, the B-efficient backcross line can produce biomass twice about the B-inefficient parent’s and show low B concentration and effective utilization of B under low B condition. Thus, the B efficiency might be attributed to the higher B utilization efficiency or less demand for B.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Jan 12, 2010

References

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