Development of an enzyme-linked diagnostic system for the detection of vitellogenin in the blood plasma of the barfin plaice Liopsetta pinnifasciata

Development of an enzyme-linked diagnostic system for the detection of vitellogenin in the blood... Vitellogenin (Vg) of the barfin plaice Liopsetta pinnifasciata was isolated and purified. Using SDS-PAGE, barfin plaice Vg was dissociated into polypeptides with molecular masses of 180, 98, 70, 52, 41, and 37 kDa. Rabbit polyclonal antibodies to this protein were obtained and a specific highly sensitive diagnostic enzyme-linked immunosorbent assay was developed for Vg detection in the blood plasma of the barfin plaice, which was tested on fish from natural habitats. The plasma Vg concentration in L. pinnifasciata from a contaminated location of Amursky Bay (Sea of Japan) in November varied from 5.295 to 28.367 mg/ml in females and from an undetectable level to 0.957 mg/ml in males. The presence of Vg in the blood plasma of male L. pinnifasciata from Amursky Bay, which was detected for the first time, is indicative of ecosystem pollution by chemicals with estrogenic activity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Marine Biology Springer Journals

Development of an enzyme-linked diagnostic system for the detection of vitellogenin in the blood plasma of the barfin plaice Liopsetta pinnifasciata

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Publisher
Springer Journals
Copyright
Copyright © 2011 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Freshwater & Marine Ecology
ISSN
1063-0740
eISSN
1608-3377
D.O.I.
10.1134/S1063074011030114
Publisher site
See Article on Publisher Site

Abstract

Vitellogenin (Vg) of the barfin plaice Liopsetta pinnifasciata was isolated and purified. Using SDS-PAGE, barfin plaice Vg was dissociated into polypeptides with molecular masses of 180, 98, 70, 52, 41, and 37 kDa. Rabbit polyclonal antibodies to this protein were obtained and a specific highly sensitive diagnostic enzyme-linked immunosorbent assay was developed for Vg detection in the blood plasma of the barfin plaice, which was tested on fish from natural habitats. The plasma Vg concentration in L. pinnifasciata from a contaminated location of Amursky Bay (Sea of Japan) in November varied from 5.295 to 28.367 mg/ml in females and from an undetectable level to 0.957 mg/ml in males. The presence of Vg in the blood plasma of male L. pinnifasciata from Amursky Bay, which was detected for the first time, is indicative of ecosystem pollution by chemicals with estrogenic activity.

Journal

Russian Journal of Marine BiologySpringer Journals

Published: Jul 8, 2011

References

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