Development of a virus-induced gene-silencing system for functional analysis of the RPS2-dependent resistance signalling pathways in Arabidopsis

Development of a virus-induced gene-silencing system for functional analysis of the... Plant Mol Biol (2007) 65:707 DOI 10.1007/s11103-007-9236-x ERRATUM Development of a virus-induced gene-silencing system for functional analysis of the RPS2-dependent resistance signalling pathways in Arabidopsis Xin-Zhong Cai Æ Qiu-Fang Xu Æ Chang-Chun Wang Æ Zhong Zheng Published online: 9 October 2007 Springer Science+Business Media B.V. 2007 Erratum to: Plant Mol Biol (2006) 62: 223–232 DOI 10.1007/s11103-006-9016-z Due to an unfortunate error, an incorrect version of Fig. 1C has been used in the above mentioned publication. The correct reproduction is published below and should be treated as definitive by the reader. Fig. 1 Effect of the Agrobacterium strain on VIGS efficiency in Arabidopsis.(C) Confirmation of the PDS gene silencing in leaves showing photo-bleaching phenotype. RT-PCR analysis was con- ducted using total RNA extracted from leaves, seen in nearly white, of plants inoculated with Agrobacterium GV3101 carrying TRV-PDS and the corresponding non-silenced leaves of the wild type TRV (TRV-00)-infected control plants. Typical PCR products are also shown for ubiquitin, used as an internal standard to correct the quantity, from the same tissues. Lanes 1-5 correspond to products from PCR of cycles 20, 24, 28, 32 and 36, respectively. Lane C represents the negative control, in which the RT reaction mix without reverse transcriptase was used as a template in the reaction The online version of the original article can be found under doi: 10.1007/s11103-006-9016-z. X.-Z. Cai (&)  Q.-F. Xu  C.-C. Wang  Z. Zheng Institute of Biotechnology, and Department of Plant Protection, Zhejiang University, 268 Kai Xuan Road, Hangzhou 310029, P.R. China e-mail: xzhcai@zju.edu.cn C.-C. Wang College of Life and Environmental Science, Zhejiang Normal University, Jinhua 321004, P.R. China http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Development of a virus-induced gene-silencing system for functional analysis of the RPS2-dependent resistance signalling pathways in Arabidopsis

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Publisher
Springer Journals
Copyright
Copyright © 2007 by Springer Science+Business Media B.V.
Subject
Life Sciences; Plant Pathology; Biochemistry, general; Plant Sciences
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-007-9236-x
Publisher site
See Article on Publisher Site

Abstract

Plant Mol Biol (2007) 65:707 DOI 10.1007/s11103-007-9236-x ERRATUM Development of a virus-induced gene-silencing system for functional analysis of the RPS2-dependent resistance signalling pathways in Arabidopsis Xin-Zhong Cai Æ Qiu-Fang Xu Æ Chang-Chun Wang Æ Zhong Zheng Published online: 9 October 2007 Springer Science+Business Media B.V. 2007 Erratum to: Plant Mol Biol (2006) 62: 223–232 DOI 10.1007/s11103-006-9016-z Due to an unfortunate error, an incorrect version of Fig. 1C has been used in the above mentioned publication. The correct reproduction is published below and should be treated as definitive by the reader. Fig. 1 Effect of the Agrobacterium strain on VIGS efficiency in Arabidopsis.(C) Confirmation of the PDS gene silencing in leaves showing photo-bleaching phenotype. RT-PCR analysis was con- ducted using total RNA extracted from leaves, seen in nearly white, of plants inoculated with Agrobacterium GV3101 carrying TRV-PDS and the corresponding non-silenced leaves of the wild type TRV (TRV-00)-infected control plants. Typical PCR products are also shown for ubiquitin, used as an internal standard to correct the quantity, from the same tissues. Lanes 1-5 correspond to products from PCR of cycles 20, 24, 28, 32 and 36, respectively. Lane C represents the negative control, in which the RT reaction mix without reverse transcriptase was used as a template in the reaction The online version of the original article can be found under doi: 10.1007/s11103-006-9016-z. X.-Z. Cai (&)  Q.-F. Xu  C.-C. Wang  Z. Zheng Institute of Biotechnology, and Department of Plant Protection, Zhejiang University, 268 Kai Xuan Road, Hangzhou 310029, P.R. China e-mail: xzhcai@zju.edu.cn C.-C. Wang College of Life and Environmental Science, Zhejiang Normal University, Jinhua 321004, P.R. China

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 9, 2007

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