Development of a reverse genetics system for the aG strain of rabies virus in China

Development of a reverse genetics system for the aG strain of rabies virus in China The aG rabies virus strain has been attenuated through multiple passages in cells and is now used as a vaccine strain in China. We attempted to develop a reverse genetics system using the aG strain. Recombinant full-length genomic cDNA was flanked by a hammerhead ribozyme and the hepatitis delta virus ribozyme. Three helper plasmids encoding the nucleoprotein, the phosphoprotein, and the large protein were produced and introduced together with a plasmid containing the full-length aG viral genome into BHK-21 cells by transfection. Recombinant virus was successfully recovered from the cloned cDNA under the control of a CMV promoter driven by RNA polymerase II. The recombinant virus was confirmed by RT-PCR, and the titer of the recombinant virus was 6.2 log LD 50 . Archives of Virology Springer Journals

Development of a reverse genetics system for the aG strain of rabies virus in China

Loading next page...
Springer Vienna
Copyright © 2014 by Springer-Verlag Wien
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
Publisher site
See Article on Publisher Site


  • Development of a reverse genetics system for a human rabies virus vaccine strain employed in China
    Huang, Y; Tang, Q; Nadin-Davis, SA
  • Initiation of Sendai virus multiplication from transfected cDNA or RNA with negative or positive sense
    Kato, A; Sakai, Y; Shioda, T; Kondo, T; Nakanishi, M; Naqai, Y
  • Structure-function relationships of hammerhead ribozyme: from understanding to applications
    Sigurdsson, ST; Eckstein, F
  • Self-cleaving ribozyme of hepatitis delta virus RNA
    Been, MD; Wickham, GS
  • An improved method for recovering rabies virus from cloned cDNA
    Inoue, KI; Shoji, Y; Kurane, I; Iijima, T; Sakai, T; Morimoto, K
  • Glycoprotein gene relocation in rabies virus
    Wu, X; Rupprecht, CE

You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial